Molecular Weight: 324.8
Storage: C, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMSO, MeOH
Absorption: (in nm) 325
Emission: (in nm) 395
Molecular Formula: C16H20N2O3.HCl
CAS Number: [62480-44-8]
Alternative Name: L-Leucine-4-methyl-7-coumarinylamide Hydrochloride
Unit Price (USD):
25 mg $110.91
Bulk Price/unit (when you buy 5 or more): $88.73
Description:
Sensitive fluorogenic substrate for leucine aminopeptidase which releases a bright blue fluorescent dye, 7-amino-4-methylcoumarin M1059 upon enzyme activity (excitation 380nm emission 440nm).
Note:
Abs = 380nm/ Em = 440nm (after leucine aminopeptidase cleavage)
Application:
Sensitivity of this enzyme using fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays.
References:
- "Profiles of bacterial community composition and metabolic potential through a plug-flow bioreactor fed with lake water." Riemann L, Sondergaard M. Journal of Plankton Research. 26(8): 973-978 (2004).
- "Comparison of different peptidase substrates for evaluation of microbial quality of aerobically stored meats." Stepaniak L. J Food Prot. 63(10): 1447-1449 (2000).
- "Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay." Irvine GB, Ennis M, Williams CH. Anal Biochem. 185(2): 304-307 (1990).
- "Subcellular localisation of leucine aminopeptidase in human polymorphonuclear leukocytes." Smith GP, MacGregor RR, Peters TJ. Biochim Biophys Acta. 728(2): 222-7 (1983).
- "Localization of leucine aminopeptidase and vitamin B-12 binding protein in rabbit peripheral blood polymorphonuclear leukocytes." Smith GP, MacGregor RR, Peters TJ. Biochim Biophys Acta. 719(3): 532-538 (1982).
- "Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin." Smith RE, Bissell ER, Mitchell AR, Pearson KW. Thromb Res. 17(3-4): 393-402 (1980).
- "A novel fluorometric ultramicro determination of serum leucine aminopeptidase using a coumarine derivative." Saifuku K, Sekine T, Namihisa T, Takahashi T, Kanaoka Y. Clin Chim Acta. 84(1-2): 85-91 (1978).
- "A new fluorogenic substrate for aminopeptidase." Kanaoka Y, Takahashi T, Nakayama H. Chem Pharm Bull (Tokyo). 25(2): 362-3 (1977).
- Methods of Enzymatic Analysis Vol. I, 26, Academic Press: New York (1975).
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