Molecular Weight: 704.97
Storage: C, D, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMF (pH > 6 )
Absorption: (in nm) 520
Emission: (in nm) 544
Extinction (103): 95
Molecular Formula: C21H7Br4NO5S
CAS Number: [60520-47-0]
Unit Price (USD):
25 mg $48.62
Bulk Price/unit (when you buy 5 or more): $38.89
Description:
Can be used as a reversible inhibitor of the erythrocyte calcium pump as well as an effective phosphorescent probe in protein rotational studies.
Note:
Eosin derivatives also exhibit phosphorescence with an emission maximum at ~680 nm.
Application:
Eosin derivatives are also used in diaminobenzidine photo-oxidation processes and fluorescence resonance energy transfer (FRET).
References:
"Avidin-EITC: an alternative to avidin-FITC in confocal scanning laser microscopy." Hulspas R, Krijtenburg PJ, Keij J.F., Bauman J.G., J. Histochem. Cytochem. 41: 1267-1272 (1993)."The Binding of Eosin-Labeled Subunit S to the Isolated Chloroplast ATPase, CF1, as Revealed by Rotational Diffusion in Solution." Wagner R., et al. FEBS Lett. 230: 109 (1988)."Chemical Modification of the Phosphoenolpyruvate Carboxylase from Maize Leaves and Its Conformation in Isotropic Solution. Studies Via Triplet Lifetime and Rotational Diffusion Using Eosin Isothiocyanate as Label." Andreo C.S., et al. Biochim Biophys Acta 870: 292 (1986)."Amino acid residues complexed with eosin 5-isothiocyanate in band 3 protein of the human erythrocyte." Chiba T., Sato Y., Suzuki Y., Biochim. Biophys. Acta 858: 107-117 (1986).
"Characteristics of anion transport channels in the human erythrocyte. I. Interactions between eosin 5-isothiocyanate and band 3 proteins." Sato Y., Chiba T., Suzuki Y., Chem. Pharm. Bull. (Tokyo) 33: 3935-3944 (1985)."Microsecond rotational motions of eosin-labeled myosin measured by time-resolved anisotropy of absorption and phosphorescence." Eads T.M., Thomas D.D., Austin R.H. J. Mol. Biol. 179: 55-81 (1984)."The reaction of fluorescein isothiocyanate with thiols: a method for assay of isothiocyanates." Wilderspin A.F., Green N.M., Anal. Biochem. 132: 449-455 (1983)."Coupling factor for photophosphorylation labeled with eosin isothiocyanate: activity, size, and shape in solution." Wagner R., Junge W. Biochemistry 21: 1890-1899 (1982)."Fluorescent triplet probes for measuring the rotational diffusion of membrane proteins." Johnson P., Garland P.B. Biochem J 203: 313-321 (1982)."Labeling of human erythrocyte membranes with eosin probes used for protein diffusion measurements: inhibition of anion transport and photo-oxidative inactivation of acetylcholinesterase." Nigg E., Kessler M., Cherry R.J., Biochim. Biophys. Acta 550: 328-340 (1979).
"Screening of Protease Inhibitors." Contillo L.G., et al. Techniques in Protein Chemistry V, Crabb J.W., Ed. pp. 493-500 (1994).
Cherry RJ, Cogoli A, Oppliger M, Schneider G, Semenza G, (1976) "A spectroscopic technique for measuring slow rotational diffusion of macromolecules. 1: Preparation and properties of a triplet probe."Biochemistry. 15(17):3653-3656.
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