Molecular Weight: 422.47
Storage: F, D
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: H2O
Molecular Formula: C17H18N2O8S2
CAS Number: [131474-38-9]
Alternative Name: D-Luciferin-6-O-β-D-galactoside
Unit Price (USD):
5 mg $157.89
Bulk Price/unit (when you buy 5 or more): $126.31
Description:
Analog of D-Luciferin contains a β-galactoside attached at the 6-O-position, and thus is not a substrate for the firefly luciferase enzyme until the galactose is removed e.g., by β-galactosidase activity. As such it represents an ultrasensitive substrate for chemiluminescent measurement of galactosidase activity in homogeneous assays, or in cell lysate samples when the enzyme luciferase is added.
Application:
This substrate represents the active component of the Beta-Glo™-type chemiluminescent galactosidase assay systems and when used as a dual substrate, β-galactosidase levels can be determined with high sensitivity and can be quantified by the release of luciferin through luciferase reaction.
References:
"Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors." Yang,Y., Janatova, J., Andrade, J.D Anal. Biochem 33: 102-107 (2005)."A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase"· Geiger R., Schneider E., Wallenfels K., Miska W. Biol. Chem. Hoppe-Seyler 373(12): 1187-91 (1992). "Bioluminescent assay of b-galactosidase using D-luciferin-o-b-galactoside." · Ugarova, N. N.; Voznyi, Ya. V.; Kutuzova, G. D.; Dementeva, E. I. Biolumin. Chemilumin. Proc. Int. Symp., 6th : 511-14 (1991). "A new homogeneous enzyme immunoassay using recombinant enzyme fragments" · Khanna, P.L. Dworschack R.T., Manning W.B., Harris J.D. Clin. Chim. Acta 33: 231-39 (1989). "CEDIA, a new homogeneous immunoassay system." · Henderson, D.R. Friedman, S.B. Harris, J.D. Manning, W.B. Zoccoli, M.A. Clin.Chem. 32: 1637-1641 (1986).
Geiger R., Schneider E., Wallenfels K., Miska W., (1992) "A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase." Biol. Chem. Hoppe Seyler. 373(12):1187-91.
Tatsumi, H., Masuda, I., Kasai, K., Sano, A., "A method for detecting Escherichia coli group bacteria." Jpn. Kokai Tokkyo Koho (1999), 4 pp. JP 11332593
Masuda-Nishimura, I., Fukuda, S., Sano, A., Kasai, K., Tatsumi, H., (2000), "Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay." Letters in Applied Microbiology 30(2): 130-135.
Fukuda, S.; Murakami, S.; Tatsumi, H. Recombinant firefly luciferase and their application for the detection of microorganisms. Bioluminescence & Chemiluminescence, Proceedings of the International Symposium, 11th, Pacific Grove, CA, United States, Sept. 6-10, 2000 (2001), Meeting Date 2000, 285-288.
Yang, X., Janatova, J., Andrade, J.D. (2005),"Homogeneous enzyme immunoassay modified for application to luminescence-based biosensors." Analytical Biochemistry 336(1), 102-107.
Wehrman, T.S., von Degenfeld, G., Krutzik, P.O., Nolan, G.P., Blau, H.M. (2006), "Luminescent imaging of b-galactosidase activity in living subjects using sequential reporter-enzyme luminescence." Nature Methods 3(4):295-301.
Sano, A., Kasai, Koichi; Tatsumi, Hiroki; Masuda, Ikuko; Tokutake, Shoichi. (2000), "Preparation of D-Luciferin-O-b-D-glucuronide derivative, and its applications to measuring b-glucuronidase activity and detecting Escherichia coli. " Jpn. Kokai Tokkyo Koho 14 pp.
Miska, W., Monsees, T., Franken, D. R., Henkel, R.,(1997) "Evaluation of the bioluminescence-enhanced zona binding assay." Bioluminescence and Chemiluminescence: Molecular Reporting with Photons, Proceedings of the International Symposium on Bioluminescence and Chemiluminescence, 9th, Woods Hole, Mass., Oct. 4-8, 1996 Meeting Date 1996, 315-318.
Keywords:
D-Luciferin-6-0-β-D-Galactopyranoside, D-Luciferin-6-0-B-D-Galactopyranoside, D-Luciferin-6-0-Beta-D-Galactopyranoside, D-Luciferin, Galactopyranoside, Marker Gene
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