Molecular Weight: 461.62
Storage: RT
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMF.DMSO, MeOH
Emission: (in nm) 430
Molecular Formula: C21H24N.C7H7O3S
CAS Number: [115534-33-3]
Unit Price (USD):
5 mg $46.30
Bulk Price/unit (when you buy 5 or more): $37.04
Description:
Fluorescent probe for membrane fluidity measurements. Especially useful in the study of monolayer dynamics of lipoproteins and similar analogous systems.
Note:
Ex=355nm
Application:
DPH and its derivatives, TMA-DPH (N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate, and DMA-DPH (1-[4-(Dimethylamino)phenyl]-6-phenylhexatriene, M1088 are cylinder-shaped and have fluorescence emission transition dipoles that are basically aligned parallel to their long molecular axis. As such, they are very sensitive to reorientation resulting from interactions with surrounding lipids. For this reason, they are used in fluorescence polarization studies of rotational motion and for membrane fluidity measurements.
References:
"Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion(MATE) transport protein." Kaatz, GW, McAleese F, Seo, SM. Antimicrobial Agents and Chemotherapy 49(5): 1857-1864 (2005)."Incorporation of fluorescent probes into PAMAM dendrimers." Domanski DM, Klajnert B, Bryszewska M. Bioelectrochemistry 63(1-2): 193-197 (2004)."Lipid phase separation correlates with activation in platelets during chilling." Tsvetkova NM. Mol Membr Biol 17: 209-18 (2000)."Reversal of multidrug resistance phenotype by surfactants: relationship to membrane lipid fluidity." Dudeja PK, Anderson KM, Harris JS, Buckingham L, Coon JS. Arch Biochem Biophys 319: 309-15 (1995)."Internalization of the lipophilic fluorescent probe trimethylamino-diphenylhexatriene follows the endocytosis and recycling of the plasma membrane in cells." Biochim Biophys Acta 1030: 73-81 (1990)."1-[4-(Trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene:synthesis, fluorescence properties, and use as a fluorescence probe of lipid bilayers." Prendergast FG, Haugland RP, Callahan PJ. Biochemistry 20: 7333-8 (1981).
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