Molecular Weight: 856.78
Storage: C, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: H2O
Emission: (in nm) 617
Molecular Formula: C46H50Cl4N8
CAS Number: [61926-22-5]
Unit Price (USD):
1 mg $126.80
Bulk Price/unit (when you buy 5 or more): $101.44
Description:
High affinity fluorescent nucleic stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement.
Note:
Ex: 487/528 nm (wo/w DNA)
Application:
The DNA binding of each ethidium homodimer molecule covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells.
References:
"Lysine-directed conjugation of ethidium homodimer to B72.3 antibody: retention of immunoreactivity but altered tumor targeting." Harapanhalli R S; Matalka K Z; Jones P L; Mahmood A; Adelstein S J; Kassis A. Nuclear medicine and biology 25(3): 267-78 (1998). "Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus." Zakharov S F; Garner M M; Chrambach A Analytical biochemistry 224(1): 195-8 (1995). "DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety." Wagner E; Cotten M; "High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange" Rye H S; Quesada M A; Peck K; Mathies R A; Glazer A N Nucleic acids research 19(2): 327-33 (1991). "A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels" Glazer A N; Peck K; Mathies R A Proceedings of the National Academy of Sciences of the United States of America 87(10): 3851-5 (1990).
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