Molecular Weight: 67000
Storage: F, D, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: H2O, aqueous buffers
Absorption: (in nm) 595
Emission: (in nm) 615
CAS Number: [N/A]
Alternative Name: Texas Red ™ Avidin
Unit Price (USD):
5 mg $142.87
Bulk Price/unit (when you buy 5 or more): $114.30
Description:
This red fluorescent protein excites at 595 nm with an emission maximum at 615 nm. Since the excitation and emission maxima are well separated from those of fluorescein, this highly fluorescent conjugate can be employed with other fluorescein or coumarin conjugates to simultaneously localize two or three antigens in the same tissue section.
Application:
Fluorescein, Texas Red™ as well as TAMRA conjugates of Avidin and Streptavidin have become increasingly useful for DNA and RNA microarray analyses, as a method of detecting biotin-labeled antibodies, or in ELISA assays. Marker Gene's Texas Red™ Avidin is a highly fluorescent conjugate of Avidin and sulforhodamine 101. Texas Red™ Avidin is ideal for flow cytometry applications using instruments equipped with dye lasers. The great affinity of avidin for D-biotin (Ka = 10-15/M) provides for a great number of applications in biochemistry (immunoassays, receptor and histochemical studies, bacteriophage inhibitions). This fluorescently labeled avidin conjugate combines stoichiometrically with biotin, having 4-biotin binding sites per avidin molecule. In addition, the Texas Red™ Avidin is a highly cationic glycoprotein that can selectively bind to a component in human and murine mast cell granules in fixed-cell preparations, and can thus be used to identify mast cells in normal and diseased human tissue without requiring a biotinylated probe. See also our Fluorescein Isothiocyanate (FITC) Avidin Conjugate, Product M1274.
References:
Alon, R., Bayer, E. A., and Wilchek, M., (1990) "Streptavidin Contains An RYD Sequence Which Mimics The RGD Receptor Domain of Fibronectin" Biochemical and Biophysical Research Communications 170: 1236-1241. Gitlin, G., Bayer, E.A. and Wilchek, M. (1987) "Studies of the biotin -binding site of avidin." Biochem. J. 242: 923-926. Chaiet, I. and Wolf, F.J. (1964). "The properties of streptavidin, a biotin -binding protein produced by Streptomycetes." Arch. Biochem. Biophys. 106: 1-5.Wood, G.S. and Warnke, R. (1981) "Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems." J. Histochem. Cytochem. 29: 1196-1204.
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