Unit Price (USD):
1 kit $222.00
Bulk Price/unit (when you buy 5 or more): $177.60
Description:
For efficient biotinylation of antibodies and other proteins. The long spacer between the biotin and the reactive group in biotin-X succinimidyl ester enhances the ability of the conjugated biotin to interact with avidin and streptavidin.
Application:
The kit contains biotin-X succinimidyl ester, Product M0783, which reacts with primary amines of proteins, antibodies or other biomolecules to form stable biotin conjugates. Also included in the kit are a gel filtration spin-column to purify the biotinylated protein from excess biotin, reagents for quantitating the degree of biotinylation and a control. This kit contains sufficient reagents for at least five biotinylation reactions of 1–5 mg each.
References:
Perrier AL, Massoulie J, Krejci E., (2002) "PRiMA: the membrane anchor of acetylcholinesterase in the brain." Neuron. 33: 275-285.
Peng HB, Xie H, Rossi SG, Rotundo RL., (1999) "Acetylcholinesterase clustering at the neuromuscular junction involves perlecan and dystroglycan." J Cell Biol. 145: 911-921.
Haugland, R. P., and Bhalgat, M. K. (1998) Preparation of avidin conjugates. Methods Mol. Biol. 80: 185-196.
Haugland, R. P., and You, W. W. (1998) Coupling of antibodies with biotin. Methods Mol. Biol. 80: 173-183.
Haugland RP; You WW (1995) Coupling of monoclonal antibodies with biotin. Methods Mol Biol. 45: 223-233.
Green, N.M. (1970). Spectrophotometric Determination of Avidin and Biotin. Methods in Enzymology. 18: 418-425.
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