Fluorescent and Luminescent Tools for Life Science
 
 

M1166 - Resorufin 7-O-methyl ether

(7-Methoxyresorufin)

M1166
 

CAS Number: 5725-89-3

Description:
One of the most popular substrates for analysis of microsomal dealkylase and cytochrome P450 enzymes.

Application:
It produces the red fluorescent dye resorufin (EX/EM 571/585 nm) upon enzyme activity. This substrate has also been extensively used to differentiate the various isozymes of the Cytochrome P450 system.

Note:
The cytochome P450's are a large group of monooxygenase enzymes responsible for the metabolism of toxins in the body. These enzymes, requiring NADPH as a cofactor and O2 as co-substrate, are located in the endoplasmic reticulum and are highly concentrated in the liver and small intestine. Additionally, Cytochrome P450's are also found in the mitochondrial membrane. P450 enzymes encompass a highly diverse "superfamily" of hemoproteins, and metabolic oxidation of chemical compounds is the main function of the cytochrome-mediated monooxygenase or mixed-function oxidase system. Cytochrome P450 is also used as a marker for the endoplasmic reticulum. The active site of cytochrome P450 contains the catalyst heme (iron-porphyrin) with a sulfur of cysteine serving as the fifth iron ligand. Although these enzymes typically have a low turnover rate, their intracellular activity can be monitored using fluorogenic alkyl ether derivatives of fluorophores including resorufin. Ex = 463 nm [EX/EM 571/585 nm of resorufin enzymatic product]

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References:
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Alternative Name: 7-Methoxyresorufin

Unit Price (USD):

5 mg $118.52


Bulk Price/unit
(5 or more):

$94.82

Quantity:

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