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Gaussia luciferase is a new reporter gene isolated from the marine copepod Gaussia princeps, and can be expressed in mammalian cells using commercially available reporter plasmids. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate (M0739) in a reaction that emits light (at 470 nm), and has considerable advantages over other reporter genes.
The MarkerGene™ Gaussia Luciferase Cellular Assay Kit contains enough reagents for 100 assays using a microtiterplate format and includes buffers, solvents and a detailed protocol for use. Gaussia Luciferase is secreted into the cell medium of cells grown in culture, therefore cell lysis is not necessary for assaying expression levels. Gaussia luciferase is reported to generate over 1000-fold higher bioluminescent signal intensity compared to Firefly and Renilla Luciferases. The substrate, coelenterazine (M0739) is common to a number of marine bioluminescent reactions, including those from Renilla, Aequorea and Watesenia.
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McCapra and Beheshti in "Bioluminescence and Chemiluminescence: Instruments and Applications," 1985, Ed. K. Van Dyke, CRC Press, Boca Raton, FL. pgs. 9-42. Schuster, G.B., Schmidt, S.P. (1982) "Chemiluminescence of Organic Compounds" Adv. Phys. Org. Chem. 18: 187. Bakhos A. Tannous, Dong-Eog Kim, Juliet L. Fernandez, Ralph Weissleder and Xandra O. Breakefield (2005) "Codon-Optimized Gaussia Luciferase cDNA for Mammalian Gene Expression in Culture and in Vivo" Molecular Therapy, 11(3): 435-443.
Verhaegent M, Christopoulos TK. (2002) "Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization." Anal. Chem. 74(17): 4378-85.
Unit Price (USD):
1 kit $75.19
(5 or more):