Fluorescent and Luminescent Tools for Life Science

2', 7'-Dichlorofluorescein di-β-D-galactopyranoside (DCFDG)

M1194
Alternative Names:Dichlorofluorescein di-Galactoside, DCFDG

Application: Highly sensitive fluorescent substrate for measuring galactosidase and galactocerebrosidase activity inside of live cells and lysosomes.

Molecular Weight: 725.48

Molecular Formula: C32H30Cl2O15


Ordering Information:
Product ID Unit Size Number of Units Price
M1194 25 mg 1-4 $87.93
    5+ $70.34

 

Description

This substrate releases the highly fluorescent fluorophore 2',7'-dichlorofluorescein (EX: 495nm / EM: 529 nm) at the site of galactosidase or galactocerebrosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores. Note: Absorption prior to enzyme hydrolysis is 290 nm; 4.7K).

Note: High Purity Grade (>99%). Absorption and Emission were measured when product released fluorophore


Technical Information

Storage: F, D, L

Soluble: DMSO, DMF, sl. H2O

Absorption: (in nm) 495

Extinction (103): 529


References:

  • van Es H.H., Veldwijk M., Havenga M., Valerio D. (1997) "A flow cytometric assay for lysosomal glucocerebrosidase" Anal. Biochem. 247: 268-271.
  • Chan KW., Waire J., Simons B., (2004) "Measurement of lysosomal glucocerebrosidase activity in mouse liver using a fluorescence-activated cell sorter assay." Anal. Biochem. 334(2): 227-33.
  • Rudensky B., Paz E., Altarescu G., (2003) "Fluorescent flow cytometric assay: a new diagnostic tool for measuring beta-glucocerebrosidase activity in Gaucher disease." Blood Cells Mol. Dis. 30(1): 97-9.
  • Daniels L.B., Glew R.H., Diven W.F., Lee R.E., Radin N.S., (1981) "An improved fluorimetric leukocyte b-glucosidase assay for Gauchers disease." Clin. Chim. Acta 115: 369-375.
  • Kaxpova E.A., Voznyi Ya V., Dudukina T.V., Tsvetkova I.V., (1991) "4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases." Biochem. Int. 24: 1135-1144.