Fluorescent and Luminescent Tools for Life Science

MarkerGene™ Long Wavelength Fluorescent Lipase Assay Kit

M1214
Application: Allows fast and easy measurement of lipase activity in vitro, in cell preparations or in vivo using the long wavelength substrate resorufin oleate.


Ordering Information:
Product ID Unit Size Number of Units Price
M1214 1 1-4 $239.00
    5+ $191.20

 

Description

Lipases are a family of enzymes that release fatty acids from triacylglycerols in a site specific manner. Most lipases have optimum activity for the primary ester groups of triglycerides, while some lipases remove fatty acyl groups from either the C-1 or C-3 acyl positions. The substrate is typically not a single molecule, but a nonaqueous phase of aggregated lipid. Lipase activity, ubiquitous among most cells, can be monitored using our long wavelength fluorescent substrate, resorufin oleate(M1208) contained in the kit. Upon cleavage, the fluorescent compound, resorufin (M0202) is released and activity measurements are easily obtained either in vitro, in cell preparations, or in vivo. The kit contains enough substrate for numerous assays and control experiments, and also contains reference standards and a detailed protocol for use. See the references below for more information and applications.


References:

  • Main LA, Okumura-Noji K, Ohnish, T, Yokoyama S. (1998) "Cholesteryl ester transfer protein reaction between plasma lipoproteins." J. Biochem. (Tokyo) 124: 237-243.
  • Jaeger KE, Ransac S, Dijkstra BW, Colson C, van Heuvel M, Misset O. (1994) "Bacterial Lipases" FEMS Microbiol. Rev. 15(1): 29-63.
  • Dousset N, Negre A, Salvayre R, Rogalle P, Dang QQ, Douste-Blazy L. (1988) "Use of a fluorescent radiolabeled triacylglycerol as a substrate for lipoprotein lipase and hepatic triglyceride lipase." Lipids 23: 605-608.
  • Galla HJ, Theilen U, Hartmann W. (1979) "Transversal Mobility in Bilayer Membrane Vesicles: Use of Pyrene Lecithin as Optical Probe" Chem Phys Lipids 23: 239-251.
  • Dergunov AD, Shuvaev VV, Perova NV. (1989) "Topo-dynamic characteristics of human plasma VLDL apolipoproteins and efficiency of triacylglycerol hydrolysis by lipoprotein lipase." Biochim Biophys Acta 1005(1): 79-86.
  • Liodakis A, Drew J, Chan RY, Sawyer WH. (1991) "Spectrofluorometric determination of lipase activity." Biochem Int 23(5): 825-34.
  • Negre A, Salvayre R, Dousset N, Rogalle P, Dang QQ, Douste-Blazy L. (1988) "Hydrolysis of fluorescent pyrenetriacylglycerols by lipases from human stomach and gastric juice." Biochim Biophys Acta 963: 340-348.
  • Hendrickson HS. (1994) "Fluorescence-bases assays of lipases, phospholipases, and other lipolytic enzymes." Analyt Biochem 219: 1-8.
  • Negre-Salvayre A, Abouakil N, Lombardo D, Salvayre R. (1990) "Hydrolysis of fluorescent pyrene-acyl esters by human pancreatic carboxylic ester hydrolase and bile salt-stimulated lipase." Lipids 25(8): 428-434.
  • Negre A, Maret A, Douste-Blazy L, Gatt S, Salvayre R. (1988) "Relative fluorescence of normal and acid lipase-deficient cultured fibroblasts following administration of pyrene decanoic acid." Biochim Biophys Acta 960: 401-409.
  • Negre A, Salvayre R, Dagan A, Gatt S. (1985) "New fluorometric assay of lysosomal acid lipase and its application to the diagnosis of Wolman and cholesteryl ester storage diseases." Clin Chim Acta 149: 81-88.
  • Rosseneu M, Taveirne M, Caster H, Van Biervliet J. (1985) "Hydrolysis of very-low-density lipoproteins labeled with a fluorescent triacylglycerol: 1,3-dioleoyl-2-(4-pyrenylbutanoyl)glycerol." Eur J Biochem 152: 195-198.
  • Mantulin WM, Massey JB, Gotto AM, Pownall HJ. (1981) "Reassembled Model Lipoproteins." J Biol Chem 256(21): 10815-10819.
  • Liodakis A, Drew J, Chan R, Sawyer WH. (1991) "Spectrofluorometric determination of lipase activity." Biochem Intern 23(5): 825-834.
  • Arpigny J L, Jaeger KE.(1999) "Bacterial lipolytic enzymes: classification and properties." Biochem J 343: 177-183.
  • Kouker G, Jaeger KE. (1987) "Specific and sensitive plate assay for bacterial lipases." Appl Environ Microbiol 53(1): 211-3.
  • Howard GT, Vicknair J, MacKie RI,. (2001) "Sensitive plate assay for screening and detection of bacterial polyurethanase activity." Lett Appl Microbiol 32(3): 211-4.
  • Salvayre R, Negre A, Radom J, Douste-Blazy L. (1986) "Fluorometric Assay for Pancreatic Lipase" Clin Chem 32(8): 1532-1536.