Molecular Weight: 445.21
Storage: RT, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: DMF, DMSO, water (neutral to high pH)
Absorption: (in nm) 504
Emission: (in nm) 529
Molecular Formula: C21H10Cl2O7
CAS Number: [111843-78-8]
Alternative Name: Carboxydichlorofluorescein, mixed isomers
Unit Price (USD):
50 mg $32.40
Bulk Price/unit (when you buy 5 or more): $25.92
Description:
Fluorescein analog with high fluorescence in the low pH range (pH 4-5). Useful for intracellular organelle staining of acidic vesicles.
Application:
Fluorescent pH indicator (pKa = 5.1).
References:
Laupeze B, Amiot L., Courtois A., Vernhet L., Drenou B., Fauchet R., Fardel O., (1999)"Use of the anionic dye carboxy-2',7'-dichlorofluorescein for sensitive flow cytometric detection of multidrug resistance-associated protein activity." Int. J. Oncol. 15:571-576.
Nedergaard M., Desai S., Pulsinelli W., (1990) "Dicarboxy-dichlorofluorescein: a new fluorescent probe for measuring acidic intracellular pH." Anal. Biochem. 187:109-114. Yin Z., Neimanis S., Heber U., (1990) "Light-Dependent pH Changes in Leaves of C3 Plants. II. Effect of CO2 and O2 on the Cytosolic and the Vacuolar pH." Planta 182, 253
"Membrane disruption by Alzheimer beta-amyloid peptides mediated through specific binding to either phospholipids or gangliosides. Implications for neurotoxicity." McLaurin J, Chakrabartty A. J Biol Chem 271, 26482-26489 (1996)
Knight C.G., Stephens T., (1989) "Xanthene-dye-labelled phosphatidylethanolamines as probes of interfacial pH. Studies in phospholipid vesicles." Biochem. J. 258: 683-689.
Zen K., Biwersi J., Periasamy N., Verkman A.S., (1992) "Second messengers regulate endosomal acidification in Swiss 3T3 fibroblasts." J Cell Biol 119:99-110.
Cotrina ML, Lin JH, Alves-Rodrigues A, Liu S, Li J, Azmi-Ghadimi H, Kang J, Naus CC, Nedergaard M. (1998) "Connexins regulate calcium signaling by controlling ATP release." Proc Natl Acad Sci U S A 95:15735-15740.
Nunnally B.K., He H., Li L.C., Tucker S.A., McGown L.B. (1997) "Characterization of visible dyes for four-decay fluorescence detection in DNA sequencing." Anal. Chem. 69:2392-2397.
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