Fluorescent and Luminescent Tools for Life Science

MarkerGene™ Fluorescent Cellulase Assay Kit


M1245
Application: Allows fast and easy detection of most cellulases in a microtiter plate based assay format. Cellulase assays are widely used in the biofuels industry.


Ordering Information:
Product ID Unit Size Number of Units Price
M1245 1 kit 1-4 $240.19
    5+ $192.15  

Description

Cellulases are a family of enzymes that include β-glucosidases, endoglucanases, and exoglucanases. These enzymes cleave the β-1,4-D-glycosidic bonds that link the glucose units comprising cellulose. In addition to being produced by plants, cellulase activity is found in many fungi and bacteria, including some plant pathogens. Most animal cells are not known to produce cellulase; cellulolytic activity is often carried out in animals by symbionts. However, recent evidence does suggest cellulase production in some animals, such as insects and arthropods. The study of cellulase activity has many applications in plant molecular biology, agriculture, and manufacturing. Cellulase is also becoming important in the development of alternative fuel sources, as glucose obtained from cellulose hydrolysis is easily fermented into ethanol. Activity of most cellulases can be monitored using our long wavelength fluorescent substrate, Resorufin Cellobioside M1238, contained in the kit. Upon cleavage, the fluorescent compound, Resorufin M0202, is released and activity measurements are easily obtained in a microtiter plate based assay format. The kit contains enough substrate for 200 assays and control experiments (100 μL reaction volume) and also contains reference standards and a detailed protocol for use. See the references below for more information and applications.

References:

  • Sizova MV, Izquierdo JA, Panikov NS, Lynd LR (2011) "Cellulose- and Xylan-Degrading Thermophilic Anaerobic Bacteria from Biocompost." Appl. Environ. Microbiol. 77(7): 2282-2291.
  • Coleman DJ, Studler MJ, Naleway JJ. (2007) "A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-β-D-cellobioside." Anal Biochem 371: 146-153.
  • Shani Z, Dekel M, Roiz L, Horowitz M, Kolosovski N, Lapidot L, Alkan S, Koltai H, Tsabary G, Goren R, Shoseyov O. (2006) "Expression of endo-1,4-β-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening." Plant Cell Rep 25: 1067-1074.
  • Villena GK, Gutierrez-Correa M. (2006) "Production of cellulase by Aspergillus niger biofilms developed on polyester cloth." Letters in Applied Microbiology. 43: 262-268.
  • Zhang Q, Bai G, Yang W, Li H, Xiong H. (2006) "Pathogenic cellulase assay of pine wilt disease and immunological localization." Biosci Biotechnol Biochem 70(11): 2727-32.
  • Nakata T, Miyafuji H, Saka S. (2006) "Bioethanol from cellulose with supercritical water treatment followed by enzymatic hydrolysis." Appl Biochem Biotechnol 129-132: 476-85.
  • Watanabe H, Tokuda G. (2001) "Animal Cellulases." Cell Mol Life Sci 58: 1167-1178.
  • Han SJ, Yoo WJ, Kang, HS.(1995)"Characterization of a Bifunctional Cellulase and Its Structural Gene." J. Biol Chem, 270(43): 26012-26019.
  • Boschker HTS, Cappenberg TE. (1994) "A sensitive method using 4-Methylumbelliferyl-beta-Cellobiose as a Substrate to Measure (1,4)-beta-Glucanase Activity in Sediments." Applied and Environmental Biology 60(10): 3592-3596.
  • Chernoglazov VM, Jafarova AN, Klyosov AA. (1989) "Continuous photometric determination of endo-1,4-beta-D-glucanase (cellulase) activity using 4-methylumbelliferyl-beta-D-cellobioside as a substrate." Anal Biochem 179(1): 186-189.
  • Thayer DW. (1978) "Carboxymethylcellulase produced by facultative bacteria from the hind-gut of the termite Reticulitermes hesperus." Journal of General Microbiology 106(1) 13-8.
  • Ferrari T, Arnison P. (1974) "Extraction and Partial Characterization of Cellulases from Expanding Pea Epicotyls." Plant Physiol 54: 487-493.
  • Paungfoo-Lonhienne C, Rentsch D, Robatzek S, Webb RI, Sagulenko E, Nšsholm T, Schmidt S, Lonhienne TG. (2010) "Turning the table: plants consume microbes as a source of nutrients." PLoS One. 5(7)
  • Sizova MV, Izquierdo JA, Panikov NS, Lynd LR. (2011) "Cellulose- and xylan-degrading thermophilic anaerobic bacteria from biocompost." Appl Environ Microbiol. 77(7):2282-91
  • Ibatullin FM, Banasiak A, Baumann MJ, Greffe L, Takahashi J, Mellerowicz EJ, Brumer H. (2009) "A real-time fluorogenic assay for the visualization of glycoside hydrolase activity in planta." Plant Physiol. 151(4):1741-50