Molecular Weight: 476.43
Storage: RT
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: MeOH, DMSO
Absorption: (in nm) 598
Emission: (in nm) 668
Molecular Formula: C29H16O7
CAS Number: [128724-35-6]
Unit Price (USD):
25 mg $48.62
Bulk Price/unit (when you buy 5 or more): $38.89
Description:
pH-dependent fluorophore with red fluorescence (EX598/EM668 nm at pH 9) and a high pKa (7.6). The fluorescence shifts to shorter wavelengths (EX512/EM567 nm) at neutral or acidic pH.
Application:
Carboxynaphthofluorescein has been exploited in both biological and industrial applications, including the construction of fiber-optic pH sensors. This long-wavelength pH indicator is also available as an amine-reactive succinimidyl ester (Product M1259) for preparing pH-sensitive protein or other conjugates.
References:
de Lamirande E, Gagnon C, (1998) "Paradoxical effect of reagents for sulfhydryl and disulfide groups on human sperm capacitation and superoxide production." Free Radical Biol. Med. 25: 803-817.
Song A, Parus S, Kopelman R, (1997) "High-performance fiber-optic pH microsensors for practical physiological measurements using a dual-emission sensitive dye." Anal. Chem. 69: 863-867.
Fabian WMF, Schuppler S, Wolfbeis OS, " (1996) in "Effects of Annulation on Absorption and Fluorescence Characteristics of Fluorescein Derivatives: a Computational Study." p. 853.
Wolfbeis OS, Rodriguez NV, Werner T,(1992) "LED-Compatible Fluorosensor for Measurement of Near-Neutral pH Values." Mikrochim. Acta 108: 133.
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