Molecular Weight: 67000

Storage: F, D, L

[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]

Soluble: H₂O, aqueous buffers

Absorption: (in nm) 494

Emission: (in nm) 518

CAS Number: [N/A]

Alternative Name: Discontinued

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Description:
This green fluorescent protein excites at 494 nm with an emission maximum at 518 nm. Since the excitation and emission maxima are well separated from those of other fluorophores such as Texas Red^TM and coumarin, FITC Avidin can be employed with other conjugates to simultaneously localize two or three antigens in the same tissue section.

Application:
Fluorescein, Texas Red^TM as well as TAMRA conjugates of Avidin and Streptavidin have become increasingly useful for DNA and RNA microarray analyses, as a method of detecting biotin-labeled antibodies, or in ELISA assays. FITC Avidin is a highly fluorescent conjugate of Avidin and fluorescein isothiocyanate. FITC Avidin is ideal for flow cytometry applications using instruments equipped with dye lasers. The great affinity of avidin for D-biotin (Ka = 10-15/M) provides for a great number of applications in biochemistry (immunoassays, receptor and histochemical studies, bacteriophage inhibitions). This fluorescently labeled avidin conjugate combines stoichiometrically with biotin, having 4-biotin binding sites per avidin molecule. In addition, the FITC Avidin is a highly cationic glycoprotein that can selectively bind to a component in human and murine mast cell granules in fixed-cell preparations, and can thus be used to identify mast cells in normal and diseased human tissue without requiring a biotinylated probe. See also Texas Red^TM Avidin Conjugate, Product M1124.

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