M1327 - pCdgCAT Mammalian CAT Expression Vector

Description:
High copy number eukaryotic vector, pCdgCAT expresses the E. coli Chloramphenicol Acetyltransferase (CAT) gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species.

Application:
The Chloramphenicol Acetyltransferase enzyme expression is enhanced by the featured Kozak's sequence and SV40 late polyadenylylation signal. pCdgCAT expression vector also contains the β-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host. In addition, the CAT gene can be excised using the XhoI/NotI sites to allow the insertion of other genes to be expressed under the same regulatory elements in mammalian cells.