Molecular Weight: 520.49
Storage: RT, L
[C=Cold D=Desiccated F=Frozen L=Light Sensitive RT=Room Temperature]
Soluble: MeOH, CH2Cl2
Absorption: (in nm) 556
Emission: (in nm) 575
Molecular Formula: C23H25IN2S2
CAS Number: [53336-12-2]
Alternative Name: DiSC3(3)
Unit Price (USD):
250 mg $41.90
Bulk Price/unit (when you buy 5 or more): $33.52
Description:
Membrane potential sensitive dye for monitoring changes in the cellular and mitochondrial membrane potential in vivo.
Application:
This dye can exhibit fluorescence changes of up to 80% upon membrane hyperpolarization (where the inside of the cell becomes more negative). The fluorescence emission and excitation (EX = 556 nm; EM = 575nm in MeOH; EX = 520 nm, EM = 595nm in water) are in the red region of the visible spectrum, allowing co-staining with other viability stains.
References:
Gaskova D, DeCorby A, Lemire BD, (2007) "DiS-C3(3) monitoring of in vivo mitochondrial membrane potential in C. elegans." Biochemical and Biophysical Research Communications 354(3): 814-819.
Sims PJ, Waggoner AS, Wang CH, Hoffman JF, (1974) "Mechanism by which cyanine dyes measure membrane potential in red blood cells and phosphatidylcholine vesicles." Biochemistry13(16): 3315-30.
Okimasu E, Kobayashi S, Aono K, Tomoda T, Utsumi K, (1981) "Tests of fluorescence changes in tumor cells due to changes in mitochondria energy metabolism with low-toxicity NK 1507." Kanko Shikiso 88: 21-30.
Denksteinova B, Sigler K, Plasek, J. 1996) "Three fluorescent probes for the flow-cytometric assessment of membrane potential in Saccharomyces cerevisiae." Folia Microbiologica (Prague) 41(3): 237-242.
Vecer J, Herman P, Holoubek A, (1997) "Diffusion membrane potential in liposomes: setting by ion gradients, absolute calibration and monitoring of fast charges by spectral shifts of diS-C3(3) fluorescence maximum." Biochimica et Biophysica Acta, Biomembranes 1325(2): 155-164.
Herman P, Vecer J, Gaskova D, Denksteinova B, Holoubek A, Plasek J, Sigler K, (1998) "Assessment of mitochondrial potential in yeasts by the fluorescent dye diS-C3(3): monitoring and modeling of membrane potential transients. Fluorescence Microscopy and Fluorescent Probes" [Based on the Proceedings of the Conference on Fluorescence Microscopy and Fluorescent Probes], 2nd, Prague, Apr. 9-12, 1997 (1998), Meeting Date 1997, 141-145.
Gaskova D, Cadek R, Chaloupka R, Plasek J, Sigler K, (2001) "Factors underlying membrane potential-dependent and -independent fluorescence responses of potentiometric dyes in stressed cells: diS-C3(3) in yeast." Biochimica et Biophysica Acta, Biomembranes 1511(1): 74-79.
Keywords:
DiSC3(3), Dipropylthiacarbocyanine iodide, Dipropylthiacarbocyanine, iodide, Marker Gene
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