Molecular Weight: 249.23
Absorption: (in nm) 395
Emission: (in nm) 561
Molecular Formula: C10H11N5O3
CAS Number: [139332-66-4]
Unit Price (USD):
10 mg $44.10
Bulk Price/unit (when you buy 5 or more): $35.28
Description:
Widely used fluorophore that has the unusual environmentally sensitive property of increasing fluorescence in low pH environments.
Application:
This common fluorescent reagent often used in carbohydrate analysis, is also useful in fluorescence visualization of lysosomes in live cell lines in culture. Comparison of staining patterns with the common LysoTracker™ or LysoSensor™ dyes indicated comparable staining, albeit using a higher concentration of NBD-piperazine (1 μM versus 100 nM for the MP dyes), but at a lower cost per assay. Marker Gene now carries this new lysosomal stain M1410 which complements our other new metabolic lysosomal enzyme probes in the MarkerGene™ LysoLive™ Lysosomal Phosphatase Assay Kit M1376 and the MarkerGene™ LysoLive™ Lysosomal Sulfatase Assay Kit M1377.
References:
Ishiguro K, Ando T, Goto H, (2008) “Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells.”BioTechniques 45(4): 465-468.
Ghosh PB, Whitehouse MW, (1968) “7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines.” Biochem. J. 108:155-156.
Toyo'oka TM, Ishibashi Y, Takeda K, Nakashima S, Akiyama S, Uzu S, Imai K, (1991) “Precolumn fluorescence tagging reagent for carboxylic acids in high-performance liquid chromatography: 4-substituted-7-aminoalkylamino-2,1,3-benzoxadiazoles.” J. Chromatogr. 588:61-71.
Toyo'oka TM, (2002) “Fluorescent tagging of physiologically important carboxylic acids, including fatty acids, for their detection in liquid chromatography.” Anal. Chim. Acta 465(1-2): 111-130.
Santa T, Matsumura D, Huang C, Kitada C, Imai K, (2002) “Design and synthesis of a hydrophilic fluorescent derivatization reagent for carboxylic acids, 4-N-(4-N-aminoethyl)piperazino-7-nitro-2,1,3-benzoxadiazole (NBD-PZ-NH2), and its application to capillary electrophoresis with laser-induced fluorescence detection.” Biomed. Chromatogr. 16:523-528.
Fehrenbacher N, Jaattela M, (2005) “Lysosomes as targets for cancer therapy.” Cancer Res. 65:2993-2995.
Kroemer G, Jaattela M, (2005) “Lysosomes and autophagy in cell death control.” Nat. Rev. Cancer 5:886-897.
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