Fluorescent and Luminescent Tools for Life Science

2', 7'-Dichlorofluorescein di-Β-D-lactoside

M1416
Application: Highly sensitive fluorescent substrate for measuring Β-lactosidase or galactosidase and glucosidase activity inside of live cells and lysosomes.

Molecular Weight: 1049.76

Molecular Formula: C44H50Cl2O25


Ordering Information:
Product ID Unit Size Number of Units Price
M1416 5mg 1-4 $112.30
    5+ $89.84

 

Description

This substrate releases the highly fluorescent fluorophore 2',7'-dichlorofluorescein (EX: 495nm / EM: 529 nm) at the site of Β-lactosidase or galactosidase and glucosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores


References:

  • Miura Y, Kim S, Etchison JR, Ding Y, Hindsgaul O, Freeze HH. (2000) "Aglycone structure influences a-fucosyltransferase III activity using N-acetyllactosamine glycoside acceptors." Glycoconjugate J 16(11):725-730.
  • Minke WE, Roach C, Hol WGJ, Verlinde CLMJ. (1999) "Structure-Based Exploration of the Ganglioside GM1 Binding Sites of Escherichia coli Heat-Labile Enterotoxin and Cholera Toxin for the Discovery of Receptor Antagonists." Biochem 38(18): 5684-5692.
  • Daniels L.B., Glew R.H., Diven W.F., Lee R.E., Radin N.S., (1981) "An improved fluorimetric leukocyte b-glucosidase assay for Gauchers disease." Clin. Chim. Acta 115: 369-375.