This substrate releases the highly fluorescent fluorophore 2',7'-dichlorofluorescein (EX: 495nm / EM: 529 nm) at the site of Β-lactosidase or galactosidase and glucosidase activity. Since the pKa of the released fluorophore is significantly lower than comparable fluorophores, it can retain appreciably more fluorescence in the highly acidic environment of the lysosome than other similar fluorophores
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