Absorption: (in nm) 485

Emission: (in nm) 520

Unit Price (USD): 1 kit $221.29

Bulk Price/unit (when you buy 5 or more): $177.03

Description:
Employs the highly sensitive substrate fluorescein di-β-D-galactopyranoside (FDG, M0250), a di-β-galactoside conjugate of the dye fluorescein to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity.

References:

• Favier, C.; Neut, C.; Mizon, C.; Cortot, A.; Colombel, J.-F.; Mizon, J. “Differentiation and identification of human fecal anaerobic bacteria producing β-galactosidase (a new methodology).” Journal of Microbiological Methods (1996), 27(1), 25-31.
• F. Russo-Marie, Roederer, M. Sager, B., Herzenberg, L., “β-Galactosidase Activity in Single Differentiating Bacterial Cells.” Proc. Natl. Acad. Sci. USA 90:8194 (1993).
• Nelis, H.; Van Poucke, S. “Enzymatic detection of coliforms and Escherichia coli within 4 hours.” Water, Air, and Soil Pollution (2000), 123(1-4): 43-52.
• Rowland B; Purkayastha A; Monserrat C; Casart Y; Takiff H; McDonough KA (1999) “Fluorescence-based detection of lacZ reporter gene expression in intact and viable bacteria including Mycobacterium species” FEMS Microbiol. Lett. 179(2): 317-25.
• Kevin L. Griffith and Richard E. Wolf, Jr. (2002) “Measuring β-Galactosidase Activity in Bacteria: Cell Growth, Permeabilization, and Enzyme Assays in 96-Well Arrays” Biochemical and Biophysical Research Communications 290: 397‾402.
• Miller, J. H. (1972) “Experiments in Molecular Genetics”, Cold Spring Harbor Laboratory Press, Cold Spring Harbor
• Ross, R. D. , Joneckis, C. C., Ordonez, J. V., Sisk, A. M., Wu, R. K., Hamburger, A. W., and Nora, R. E. (1989). “Estimation of cell survival by flow cytometric quantification of fluorescein diacetate/propidium iodide viable cell number.” Cancer Research. 49: 3776 - 3782.
• Tao He, Marion G. Priebe, Roel J. Vonk, Gjalt W. Welling. (2005) “Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects.” FEMS Microbiology Ecology 54: 463-469.