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Description: Employs the highly sensitive substrate FDG, M0250,
a di-β-galactoside conjugate of the dye fluorescein, to more effectively detect E. Coli and other coliforms through both basal and induced β-galactosidase activity.
View Technical Information ▼
Absorption: (in nm) 485
Emission: (in nm) 520
References: View References ▼
- Favier, C.; Neut, C.; Mizon, C.; Cortot, A.; Colombel, J.-F.; Mizon, J. “Differentiation and identification of human fecal anaerobic bacteria producing β-galactosidase (a new methodology).” Journal of Microbiological Methods (1996), 27(1), 25-31.
- F. Russo-Marie, Roederer, M. Sager, B., Herzenberg, L., “β-Galactosidase Activity in Single Differentiating Bacterial Cells.” Proc. Natl. Acad. Sci. USA 90:8194 (1993).
- Nelis, H.; Van Poucke, S. “Enzymatic detection of coliforms and Escherichia coli within 4 hours.” Water, Air, and Soil Pollution (2000), 123(1-4): 43-52.
- Rowland B; Purkayastha A; Monserrat C; Casart Y; Takiff H; McDonough KA (1999) “Fluorescence-based detection of lacZ reporter gene expression in intact and viable bacteria including Mycobacterium species” FEMS Microbiol. Lett. 179(2): 317-25.
- Kevin L. Griffith and Richard E. Wolf, Jr. (2002) “Measuring β-Galactosidase Activity in Bacteria: Cell Growth, Permeabilization, and Enzyme Assays in 96-Well Arrays” Biochemical and Biophysical Research Communications 290: 397‾402.
- Miller, J. H. (1972) “Experiments in Molecular Genetics”, Cold Spring Harbor Laboratory Press, Cold Spring Harbor
- Ross, R. D. , Joneckis, C. C., Ordonez, J. V., Sisk, A. M., Wu, R. K., Hamburger, A. W., and Nora, R. E. (1989). “Estimation of cell survival by flow cytometric quantification of fluorescein diacetate/propidium iodide viable cell number.” Cancer Research. 49: 3776 - 3782.
- Tao He, Marion G. Priebe, Roel J. Vonk, Gjalt W. Welling. (2005) “Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects.” FEMS Microbiology Ecology 54: 463-469.
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Unit Price (USD):
1 kit $232.35
Bulk Price/unit (5 or more): $185.88
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