Molecular Weight: 631.68
Absorption: (in nm) 578
Emission: (in nm) 600
Molecular Formula: C37H33N3O7
CAS Number: [216699-36-4]
Alternative Name: 6-ROX, NHS
Unit Price (USD):
5 mg $200.03
Bulk Price/unit (when you buy 5 or more): $160.02
Description:
This dye is commonly used in dye-terminator sequencing methods by attachment to dideoxynucleotide triphosphates (ddNTP's). It can also be used in other DNA or RNA labeling methods.
References:
Mackay IM (2004) "Real-time PCR in the microbiology laboratory." Clin. Microbiol. Infect. 10(3): 190-212.
Ju J, Ruan C, Fuller CW, Glazer AN, Mathies RA (1995) "Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis." Proc. Natl. Acad. Sci. USA (92):10 4347-4351.
Ansorge W, Posenthal A, Sproat B, Stegemann J, Voss H, (1988) "Non-radioactive automated sequencing of oligonucleotides by chemical degradation." Nucleic Acids Res. 16: 2203-2206.
Prober JM, Trahnor SH, Dann RJ, Hobbs FW, Robertson CW, Zagursky RJ, Cocuzza AJ, Jensen MA, Baumeister K (1987) "A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides." Science 238: 336-341.
Smith LM, Fung S, Hunkapiller MW, Hunkapiller TJ, Hood LE (1986) "The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis." Nucleic Acids Res. 13: 2399-2412.
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