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M1475 - Marker Gene™ Non-Radioactive Cell Proliferation Assay Kit
(MTT Assay Kit)

Alternative Name: MTT Assay Kit

Unit Price (USD): 1 Kit $224.00

Bulk Price/unit (when you buy 5 or more): $179.20

Description:

The MarkerGene™ Non-Radioactive Cell Proliferation Assay Kit uses a common tetrazolium dye MTT ((3-(4,5-imethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as a method of quickly and accurately measuring cell viability. The assay relies on the reduction by live cells of the tetrazolium salt MTT by intracellular oxidoreductases. It is therefore widely regarded as an accurate indicator of cell redox activity.

References:

  • Mosmann, T (December 1983). "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays". Journal of immunological methods 65 (1-2): 55–63. PMID 6606682.
  • Denizot F, Lang R 1986. “Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and readability.” J Immunol Meth 89: 271-277.
  • Carmichael, James; DeGraff, William G.; Gazdar, Adi F.; Minna, John D.; Mitchell, James B. “Evaluation of a tetrazolium - based semiautomated colorimetric assay: assessment of radiosensitivity.” Cancer Research (1987), 47(4), 943-6.
  • Alley M C; Scudiero D A; Monks A; Hursey M L; Czerwinski M J; Fine D L; Abbott B J; Mayo J G; Shoemaker R H; Boyd M R “Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.” Cancer research (1988), 48(3), 589-601. Journal code: 2984705R.
  • Rubinstein, L. V.; Shoemaker, R. H.; Paull, K. D.; Simon, R. M.; Tosini, S.; Skehan, P.; Scudiero, D. A.; Monks, A.; Boyd, M. R. “Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines.” Journal of the National Cancer Institute (1990), 82(13), 1113-18.
  • Scudiero, Dominic A.; Shoemaker, Robert H.; Paull, Kenneth D.; Monks, Anne; Tierney, Siobhan; Nofziger, Thomas H.; Currens, Michael J.; Seniff, Donna; Boyd, Michael R. “Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. “ Cancer Research (1988), 48(17), 4827-33.
  • Gonzalez, Basilio; Akman, Steve; Doroshow, James; Rivera, Hector; Kaplan, William D.; Forrest, Gerald L. “Protection against daunorubicin cytotoxicity by expression of a cloned human carbonyl reductase cDNA in K562 leukemia cells.” Cancer Research (1995), 55(20), 4646-50.
  • Marks, Denese C.; Belov, Larissa; Davey, Mary W.; Davey, Ross A.; Kidman, Antony D. “The MTT cell viability assay for cytotoxicity testing in multidrug-resistant human leukemic cells.” Leukemia Research (1992), 16(12), 1165-73.
  • Mitochondrial and nonmitochondrial reduction of MTT: interaction of MTT with TMRE, JC-1, and NAO mitochondrial fluorescent probes. Bernas T, Dobrucki J. Cytometry. 2002 Apr 1;47(4):236-42.



    • Keywords:

    Kit, MTT, Assay, Formazan, Non-radioactive, Proliferation Assay, Marker Gene


©2010 Marker Gene Technologies, Inc. Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA. All rights reserved. For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.