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Description:
This kit uses the MTT dye to measure cell viability.
Application:
The MarkerGene™ Non-Radioactive Cell Proliferation Assay Kit uses the common tetrazolium dye MTT to quickly and accurately measure cell viability. The assay relies on the reduction of MTT by intracellular oxidoreductases in live cells. The absorbance of this colored solution is then quantified using a plate reader or spectrophotometer with absorbance reading at 550-600 nm. A very commonly used method, the MTT assay has been referenced over 10,000 times in PubMed.
The MarkerGene™ Non-Radioactive Cell Proliferation Assay Kit provides sufficient reagents for 2500 assays of 100 ul each.
References:
View References ▼
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Mosmann, T (December 1983). "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays". Journal of immunological methods 65 (1-2): 55–63. PMID 6606682.
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Denizot F, Lang R 1986. “Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and readability.” J Immunol Meth 89: 271-277.
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Carmichael, James; DeGraff, William G.; Gazdar, Adi F.; Minna, John D.; Mitchell, James B. “Evaluation of a tetrazolium - based semiautomated colorimetric assay: assessment of radiosensitivity.” Cancer Research (1987), 47(4), 943-6.
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Alley M C; Scudiero D A; Monks A; Hursey M L; Czerwinski M J; Fine D L; Abbott B J; Mayo J G; Shoemaker R H; Boyd M R “Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.” Cancer research (1988), 48(3), 589-601. Journal code: 2984705R.
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Rubinstein, L. V.; Shoemaker, R. H.; Paull, K. D.; Simon, R. M.; Tosini, S.; Skehan, P.; Scudiero, D. A.; Monks, A.; Boyd, M. R. “Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines.” Journal of the National Cancer Institute (1990), 82(13), 1113-18.
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Scudiero, Dominic A.; Shoemaker, Robert H.; Paull, Kenneth D.; Monks, Anne; Tierney, Siobhan; Nofziger, Thomas H.; Currens, Michael J.; Seniff, Donna; Boyd, Michael R. “Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. “ Cancer Research (1988), 48(17), 4827-33.
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Gonzalez, Basilio; Akman, Steve; Doroshow, James; Rivera, Hector; Kaplan, William D.; Forrest, Gerald L. “Protection against daunorubicin cytotoxicity by expression of a cloned human carbonyl reductase cDNA in K562 leukemia cells.” Cancer Research (1995), 55(20), 4646-50.
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Marks, Denese C.; Belov, Larissa; Davey, Mary W.; Davey, Ross A.; Kidman, Antony D. “The MTT cell viability assay for cytotoxicity testing in multidrug-resistant human leukemic cells.” Leukemia Research (1992), 16(12), 1165-73.
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Mitochondrial and nonmitochondrial reduction of MTT: interaction of MTT with TMRE, JC-1, and NAO mitochondrial fluorescent probes. Bernas T, Dobrucki J. Cytometry. 2002 Apr 1;47(4):236-42.
Alternative Name: MTT Assay Kit
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Unit Price (USD):
1 Kit $246.96
Bulk Price/unit
(5 or more): $197.57
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