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Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) for measurement of cell proliferation based upon the generation of ATP in metabolically active cells.
Useful for multiplexing. The kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
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Cunningham BA. (2001) “A growing issue: cell proliferation assay” The Scientist 15(13): 26.
Tatsumi H, Masuda T, Kajiyama N, Nakano E. (1989) "Luciferase cDNA from Japanese firefly, Luciola cruciata: cloning, structure and expression in Escherichia coli." J Biolumin Chemilumin 3(2): 75-78.
Masuda T, Tatsumi H, Nakano E. (1989) "Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly, Luciola cruciata." Gene 77(2): 265-270.
de Wet JR, Wood KV, Helinski DR, DeLuca M. (1985) "Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli." Proc Natl Acad Sci U S A. 82(23): 7870-7873.
Mamaev SV, Laikhter AL, Arslan T, Hecht SM (1996) "Firefly Luciferase: Alteration of the Color of Emitted Light Resulting from Substitutions at Position 286." J. Amer. Chem. Soc. 118: 7243-7244.
Kajiyama N, Nakano E. (1991) "Isolation and characterization of mutants of firefly luciferase which produce different colors of light." Protein Eng. 4: 691.
Kajiyama N, Nakano E. (1993) "Thermostabilization of Firefly Luciferase by a Single Amino Acid Substitution at Position 217." Biochem 32: 13795-13799.
Crouch SP, Kozlowski R, Slater KJ, Fletcher J. (1993) "The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity," J. Immunol. Methods 160(1): 81-88.
Unit Price (USD):
1 kit $115.50
(5 or more):