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Description:
Unlike other commercially available luciferase kits using green light emitting (562 nm) firefly analogs, this luminescent assay kit produces a red light (EM 619 nm) for measurement of cytotoxicity based upon the generation of ATP in metabolically active cells.
Application:
Useful for multiplexing. The kit components involve no radioisotopes or toxic materials and are environmentally safe. Each kit provides enough reagents and a detailed protocol sufficient for 100 reactions in 96-well microplate format.
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References:
View References ▼
Cunningham BA. (2001) “A growing issue: cell proliferation assay” The Scientist 15(13): 26.
Tatsumi H, Masuda T, Kajiyama N, Nakano E. (1989) "Luciferase cDNA from Japanese firefly, Luciola cruciata: cloning, structure and expression in Escherichia coli." J Biolumin Chemilumin 3(2): 75-78.
Masuda T, Tatsumi H, Nakano E. (1989) "Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly, Luciola cruciata." Gene 77(2): 265-270.
de Wet JR, Wood KV, Helinski DR, DeLuca M. (1985) "Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli." Proc Natl Acad Sci U S A. 82(23): 7870-7873.
Mamaev SV, Laikhter AL, Arslan T, Hecht SM (1996) "Firefly Luciferase: Alteration of the Color of Emitted Light Resulting from Substitutions at Position 286." J. Amer. Chem. Soc. 118: 7243-7244.
Kajiyama N, Nakano E. (1991) "Isolation and characterization of mutants of firefly luciferase which produce different colors of light." Protein Eng. 4: 691.
Kajiyama N, Nakano E. (1993) "Thermostabilization of Firefly Luciferase by a Single Amino Acid Substitution at Position 217." Biochem 32: 13795-13799.
Crouch SP, Kozlowski R, Slater KJ, Fletcher J. (1993) "The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity," J. Immunol. Methods 160(1): 81-88.
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Unit Price (USD):
1 kit $115.50
Bulk Price/unit
(5 or more): $115.50
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