Fluorescent and Luminescent Tools for Life Science

pCambia1381Xa Plant Expression Vector


M1599
Application: Vector contains kanamycin resistance gene for bacterial selection and hygromycin B resistance gene for plant selection. Also incorporates the gusA reporter gene with no start codon to allow fusion of your gene to gusA with reading frame a.


Ordering Information:
Product ID Unit Size Number of Units Price
M1599 20 μg 1-4 $114.45
    5+ $114.45  

Description

This vector is used for the transfection of plants with exogenous genes to study their function in planta.

Note:

This product is prepared to order. Please allow additional processing time prior to shipping.

GenBank Sequence Data

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References:

  • Chen L, Zhang S, Beachy RN, Fauquet CM (1998) “A protocol for consistent, large-scale production of fertile transgenic rice plants.” Plant Cell Reports 18:25-31.
  • Christou P (1991) “Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos.” Biotechnology 9:957-962.
  • Christou P (1997) “Rice transformation: bombardment”. Plant Mol Biol 35:197-203. Deblaere R, Reynaerts A, Hofte H, Hernalsteens JP, Leemans J, Van Montagu M (1987) “Vectors for cloning in plant cells.” Meth Enzymol 153:277-292.
  • Hajdukiewicz,P, Svab, Z, Maliga, P, (1994) “The small versatile pPZP family of Agrobacterium binary vectors for plant transformation.” Plant Mol Biol 25:989-994.
  • Hiei Y, Ohta S, Komari T, Kumashiro T (1994) “Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.” Plant J 6:271-282.
  • Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) “Binary vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid.” Nature 303:179-180.
  • Hood EE, Helmer GL, Fraley RT, Chilton MD (1986) “The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.” J Bac 168:1291-1301.
  • Hood EE, Gelvin SB, Melchers S, Hoekema A (1993) “New Agrobacterium helper plasmids for gene transfer to plants (EHA105).” Trans Res 2:208-218.
  • Jefferson RA, Kavanagh TA, Bevan MW (1987) “GUS fusions: Beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.” EMBO J 6:3901-3907.
  • Klapwijk PM, van Breukelen J, Korevaar K, Ooms G, Schilperoort RA (1980) “Transposition of Tn904 encoding streptomycin resistance into octopine Ti plasmid of Agrobacterium tumefaciens.” J Bac 141:129-136.
  • Lazo GR, Stein PA, Ludwig RA (1991) “A DNA transformation-competent Ara bidopsis genomic library in Agrobacterium.” BioTechnology 9:963-967.
  • Ohta S, Mita S, Hattori T, Nakamura K (1990) “Construction and expression in tobacco of a beta-glucuronidase (GUS) reporter gene containing an intron within the coding sequence.” Plant Cell Physiol 31:805-814.
  • Ooms G, Hooykaas PJJ, Van Veen RJM, Van Beelen P, Regensburg-Tunk JG, Schilperoort RA (1982) “Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region:. Plasmid 7 :15-29.
  • Peralta EG, Hellmiss R, Ream W (1986) “Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid.” EMBO J 5:1137-1142.
  • Porath J (1992) “Immobilized metal ion affinity chromatography.” Protein Expre Purif 3:263-281.
  • Siemering KR, Golbik R, Sever R, Haseloff J (1996) “Mutations that suppress the thermosensitivity of green fluorescent protein.” Curr Biol 6:1653-1663.
  • Tanaka A, Mita S, Ohta S, Kyozuka J, Shimamoto K, Nakamura K (1990) “Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron.” Nucl Acids Res 18:6767-6770.
  • http://www.cambia.org/daisy/cambia/585.html#dsy585_Description