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Fluorescence in situ hybridization (FISH) kit which directly labels the phosphate groups (terminal and backbone) on DNA using a TAMRA labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.
The supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid. Standard size kit is suitable for labeling up to 120 μg of nucleic acid.
View References ▼
Durrant I, Brunning S, Eccleston L, Chadwick P, Cunningham M. (1995) "Fluorescein as a label for non-radioactive in situ hybridization." Histochem J 27:94-99.
Forster AC, McInnes JL, Skingle DC, Symons RH. (1985) "Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin." Nucleic Acids Res 13: 745-791.
Hoevel T, and Kubbies M. (2002) "Nonradioactive labeling and detection of mRNAs hybridized onto nucleic acid cDNA arrays." Methods Mol Biol 185: 417-23.
Kessler C. (1995) "Methods for nonradioactive labeling of nucleic acids." In Nonisotopic probing, blotting, and sequencing (Kricka L J, Ed.) pp 41-109, Academic Press, San Diego.
Liu P, Siciliano J, Seong D, Craig J, Zhao Y, Jong P, Siciliano M. (1993) "Dual Alu polymerase chain reaction primers and conditions for isolation of human chromosome painting probes from hybrid cells." Cancer Genetics Cytogenetics 65:93-99.
Mannot V, Tora C, Lopez S, Menou L, Laayoun A. (2001) "Labeling during cleavage (LDC), a new labeling approach for RNA." Nucleosides, Nucleotides, Nucleic Acids 20: 1177-1179.
Olejnik J, Krymanska-Olejnik E, Rothschild KJ. (1998) "Photocleavable aminotag phosphoramidites for 5'-termini DNA/RNA labeling" Nucleic Acids Res 26(15): 3572-3576.
Rihn H, Coulais C, Bottin MC, Martinet N. (1995) "Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids: Part one: chemiluminescent methods." Biochem Biophys Methods 30:91-102.
Sambrook J, Fritsch EF, Maniatis T (1989) "Molecular Cloning: A Laboratory Manual." 2nd edition.
Henegariu O, Bray-Ward P, Artan S, Vance G, Qumsyieh M, Ward D. (2001) "Small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH)." Lab Investig 81(4): 475-481.
Weier H, Lucas J, Poggensee M, Segraves R, Pinkel D, Gray J. (1991) "Two-color hybridization with high complexity chromosome-specific probes and a degenerate alpha satellite probe DNA allows unambiguous discrimination between symmetrical and asymmetrical translocations." Chromosoma 100:371-376.
Unit Price (USD):
1 kit $756.00
(5 or more):