Fluorescent and Luminescent Tools for Life Science

OliGlo™Cy3 FISH Kit - standard size


M1623
Application: Fluorescence in situ hybridization (FISH) kit which directly labels the phosphate groups (terminal and backbone) on DNA using a Cy3 labeling reagent prepared in situ, causing less disruption of hybridization and allowing labeling that is not sequence dependent.


Ordering Information:
Product ID Unit Size Number of Units Price
M1623 1 kit 1-4 $756.00
    5+ $756.00  

Description

The supplied standard labeling protocol will yield labeling efficiency of approximately 10 to 100 labels per kilobase of nucleic acid. Standard size kit is suitable for labeling up to 120 μg of nucleic acid.

References:

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  • Forster AC, McInnes JL, Skingle DC, Symons RH. (1985) "Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin." Nucleic Acids Res 13: 745-791.
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  • Olejnik J, Krymanska-Olejnik E, Rothschild KJ. (1998) "Photocleavable aminotag phosphoramidites for 5'-termini DNA/RNA labeling" Nucleic Acids Res 26(15): 3572-3576.
  • Rihn H, Coulais C, Bottin MC, Martinet N. (1995) "Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids: Part one: chemiluminescent methods." Biochem Biophys Methods 30:91-102.
  • Sambrook J, Fritsch EF, Maniatis T (1989) "Molecular Cloning: A Laboratory Manual." 2nd edition.
  • Henegariu O, Bray-Ward P, Artan S, Vance G, Qumsyieh M, Ward D. (2001) "Small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH)." Lab Investig 81(4): 475-481.
  • Weier H, Lucas J, Poggensee M, Segraves R, Pinkel D, Gray J. (1991) "Two-color hybridization with high complexity chromosome-specific probes and a degenerate alpha satellite probe DNA allows unambiguous discrimination between symmetrical and asymmetrical translocations." Chromosoma 100:371-376.