Unit Price (USD): 20μg $250.00

Description:
High copy number eukaryotic vector, pFHluc, expresses a mutant luciferase gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Contains a multiple cloning site with 11 unique restriction sites. This vector encodes a mutant enzyme that catalyzes the production of long wavelength (red) light (Em:619) from D-luciferin, M0237.

Application:
This mutant luciferase is optimized for expression in mammalian cells, resulting in drastically improved light production, allowing detection of expression at extremely low levels. The mutant luciferase also has been shown to remain stable at 37°C over long time periods, a characteristic not seen in wild type luciferases. This vector is very useful for transfection of mammalian cells in culture and may also be used in other species. The mutant luciferase enzyme expression is enhanced by the Kozak's consensus sequence and SV40 late polyadenylylation signal. The pFHluc expression vector also contains the ß-lactamase gene, which acts as a selection marker (100μg/mL ampicillin resistance) in E. coli host.

References:

• Tatsumi H, Masuda T, Kajiyama N, Nakano E (1989) "Luciferase cDNA from Japanese firefly, Luciola cruciata: cloning, structure and expression in Escherichia coli." J. Biolumin. Chemilumin. 3(2):75-78.
• Masuda T, Tatsumi H, Nakano E (1989) "Cloning and sequence analysis of cDNA for luciferase of a Japanese firefly, Luciola cruciata." Gene. 77(2):265-270.
• de Wet JR, Wood KV, Helinski DR, DeLuca M (1985) "Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli." Proc Natl Acad Sci U S A. 82(23):7870-7873.
• Mamaev SV, Laikhter AL, Arslan T, Hecht SM, (1996) "Firefly Luciferase: Alteration of the Color of Emitted Light Resulting from Substitutions at Position 286."
• Kajiyama, N, Nakano E, (1991) "Isolation and characterization of mutants of firefly luciferase which produce different colors of light." Protein Eng. 4: 691.
• Kajiyama N, Nakano E, (1993) "Thermostabilization of Firefly Luciferase by a Single Amino Acid Substitution at
  Position 217." Biochemistry 32: 13795-1 3799.