Enzyme Substrates and Kits for Protease Enzymes.

Proteases are a family of enzymes that cleave proteins and peptides at site-specific peptide linkages within their structures. They are further classified as either endopeptidases, which cleave proteins or peptides at internal peptide linkages, or exopeptidases, which can cleave the terminal amino-acid residues from peptides or proteins. Marker Gene sells a variety of chromogenic, fluorogenic or secondary enzyme specific protease substrates or kits for use in assay development and research. Many of these substrates and kits are specific for important intracellular enzymes such as the Caspases, Calpains, Cathepsins used in apoptosis reasearch. Please see the list below for more information about individual products.

Product ID Product Name Description
M0353 N-CBZ-ß-Glycinyl-6-Aminoluciferin  This protected glycine derivative produces the chemiluminescent compound 6-Amino-D-Luciferin Product M0352 upon peptidase enzymatic reaction. Subsequent analysis using the firefly luciferase detection system, provides an ultrasensitive method of measuring peptidase activity in vitro. Aminoluciferin is a very sensitive substrate of firefly luciferase and can be easily quantified in a luminometric assay. 
M0815 FAM Poly Caspases Assay Kit 25 tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0816 FAM Poly Caspases Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0817 FAM Caspase 1 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0818 FAM Caspase 1 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0819 FAM Caspase 2 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0820 FAM Caspase 2 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0821 FAM Caspases 3 and 7 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0822 FAM Caspases 3 and 7 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0823 FAM Caspase 6 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0824 FAM Caspase 6 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0825 FAM Caspase 8 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0826 FAM Caspase 8 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0827 FAM Caspase 9 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0828 FAM Caspase 9 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0829 FAM Caspase 10 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0830 FAM Caspase 10 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0831 FAM Caspase 13 Assay Kit 25tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0832 FAM Caspase 13 Assay Kit 100tests  FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly. The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.  
M0833 Sulforhodamine Poly Caspases Detection Kit 25tests  SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases. The methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA inhibitor binds covalently to the active caspase. The SR FLICA reagent excites at 550 nm and has a maximum emission range of 590 – 600 nm. 
M0834 Sulforhodamine Poly Caspases Detection Kit 100tests  SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases. The methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA inhibitor binds covalently to the active caspase. The SR FLICA reagent excites at 550 nm and has a maximum emission range of 590 – 600 nm. 
M0835 Sulforhodamine Caspases 3 and 7 Assay Kit 25tests  SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases. The methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA inhibitor binds covalently to the active caspase. The SR FLICA reagent excites at 550 nm and has a maximum emission range of 590 – 600 nm. 
M0836 Sulforhodamine Caspases 3 and 7 Assay Kit 100tests  SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases. The methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA inhibitor binds covalently to the active caspase. The SR FLICA reagent excites at 550 nm and has a maximum emission range of 590 – 600 nm. 
M0837 Magic RedTM-(DEVD)2 Caspases 3 and 7Assay Kit 25tests  Magic Red™ products are substrate-based assays designed to detect protease activity within whole living cells, using a fluorescence microscope. Assay kits are available in 25-test and 100-test sizes.  
M0838 Magic RedTM-(DEVD)2 Caspases 3 and 7Assay Kit 100tests  Magic Red™ products are substrate-based assays designed to detect protease activity within whole living cells, using a fluorescence microscope. Assay kits are available in 25-test and 100-test sizes.  
M0839 Magic RedTM-(RR)2 Cathepsin B Assay Kit 25tests  These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. 
M0840 Magic RedTM-(RR)2 Cathepsin B Assay Kit 100tests  These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. 
M0841 Magic RedTM-(LR)2 Cathepsin K Assay Kit 25tests  These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. 
M0842 Magic RedTM-(LR)2 Cathepsin K Assay Kit 100tests  These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. 
M0843 Magic RedTM-(FR)2 Cathepsin L Assay Kit 25tests  These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. 
M0844 Magic RedTM-(FR)2 Cathepsin L Assay Kit 100tests  These Magic Red™ substrate-based assays are designed to detect Cathepsin protease activity within whole living cells, using fluorescence microscopy. 
M0845 MitoPTTM Apoptosis Detection Kit 25tests  Use of the MitoPT™ kit allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells. 
M0846 MitoPTTM Apoptosis Detection Kit 100tests  Use of the MitoPT™ kit allows the easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells. 
M1060 L-Leucine 7-amido-4-methyl coumarin Hydrochloride (Leu-AMC.HCl)  This sensitive fluorogenic substrate for leucine aminopeptidase releases a bright blue fluorescent dye, 7-amino-4-methylcoumarin Product M1059 upon enzyme activity (excitation 380nm emission 440nm). Sensitivity of this enzyme using fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays. 
M1319 MarkerGeneTM Fluorescent Protease Assay Kit  The Fluorescent Protease Assay Kit allows quick and simple measurement of general protease activity in biological samples.  
     
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