Monthly Newsletter Archives

© Copyright MGT, Inc., 2007.  Published by Marker Gene Technologies, Inc., The University of Oregon Riverfront Research Park, 1850 Millrace Drive, Eugene, Oregon 97403-1992 USA.  All rights reserved.  For information on the use or copying of the material contained in this document, please contact us at techservice@markergene.com.  Please see below for subscription information and updates.

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Volume 8, Number 7: July, 2008 

  • BrdU Alternatives for Cell Proliferation Assays.
  • a-Mannosidase Assays for Golgi Mannosidase II Analysis.
  • FACS Blue lacZ ß-Galactosidase Detection Kit.
  • Apoptosis Induction Assays.

Volume 8, Number 6: June, 2008 

  • Measuring Oxidative Stress in Cells.
  • New Senescence Assay Kit.
  • More Photostable Fluorescent Proteins.
  • The Nitroreductase Gene Reporter System.

Volume 8, Number 5: May, 2008 

  • Glycan Development in Zebrafish Embryos.
  • Enzyme Processing in the Golgi Apparatus.
  • Pyrenedecanoic Acid for Oxygen Measurements.
  • GUS Reporter Gene Vectors and Detection in Plants.

Volume 8, Number 4: April, 2008 

  • New Metabolic Lysosomal Enzyme Probes.
  • Sulfatase Inhibitor Assays for P-Selectin Binding.
  • Intracellular Biosensor Technology.
  • ß-Lactamase Detection Using Nitrocephin.

Volume 8, Number 3: March, 2008 

  • Cellulase assay as a method of monitoring grape ripening.
  • Metabolic Lysosomal Enzyme Probes.
  • Mammalian Luciferase Detection Vectors and Applications.

Volume 8, Number 2: February, 2008 

  • Luciferase/GUS/GFP Enhancer Trap System in Plants.
  • Biotin Analysis Systems.
  • Chromogenic Mammalian ß-Gal Staining Methods.
  • Quantum Dots with Reduced Toxicity.

Volume 8, Number 1: January, 2008 

  • Intestinal Alkaline Phosphatase Inhibition of LPS Toxicity.
  • Lipoic Acid Ligase for Cell Surface Protein Labeling.
  • Neomycin Phosphotransferase Selection of Pluripotent Stem Cells.
  • Mitochondrial Potential Measurements.

Volume 7, Number 12: December, 2007 

  • Hypoxia in Tumor Tissues Measured Using Marker Genes.
  • Transgenic Feline Model with Ubiquitous Red Fluorescent Protein Expression.
  • Carbohydrate Analysis by Fluorescence Derivatization.
  • Mitochondrial and Cellular Membrane Potential Measurements Using DiS-C3(3).
  • Visit us at the Experimental Biology and Cell Biology Meetings.

Volume 7, Number 11: November, 2007 

  • Methicillin Resistant Staphylococcus aureus (MRSA) Enzyme Assays.
  • Labeling with MRI Contrast Agents.
  • Bronchoalveolar Eosinophil Staining with FITC.
  • a-Mannosidase Measurement in Mucolipidosis and Dystrophy.

Volume 7, Number 10: October, 2007 

  • HSV1-TK Marker Gene Assays.
  • New Fluorescent Protease Assay Kit.
  • Chloramphenicol Acetyl Transferase (CAT) Assay.
  • Cytotoxicity Testing.

Volume 7, Number 9: September, 2007 

  • Bioluminescence Resonance Energy Transfer (BRET)
  • GFP Blinking used as a Molecular Thermometer.
  • Amylase Determination Using Long-Wavelength Detection.
  • Fluorescent Protease Assays.

Volume 7, Number 8: August, 2007 

  • Whole Genome Transplant – Toward a Synthetic Life Form.
  • Bacterial Luciferase Assay in Mammalian Cells.
  • Live Cell FDG Plate Assay for Mammalian Cells.
  • Serum Lipase Assays.

Volume 7, Number 7: July, 2007 

  • Heat-Shock Promoter Elements.
  • Selective PMCA Inhibitor of Calcium Clearance.
  • A Novel, Water Soluble Lipase Substrate.
  • Long-Wavelength Esterase Activity Assays.

Volume 7, Number 6: June, 2007 

  • LacZ and neoR used to Identify Stem Cells Generated from Mouse Fibroblasts.
  • a-(1-4)-di-Galacturonic Acid.
  • A New Zinc-Binding BODIPY Probe.
  • New Thiol Reactive Probes.
  • Order Using our New e-Commerce System.

Volume 7, Number 5: May, 2007 

  • GFP Complementation Assays for RNA analysis.
  • DiI(C18:5) as a Long-Term Membrane Probe and Counterstain.
  • Combined Rose Bengal - Luciferase System for Photodynamic Therapy
  • Carboxyfluorescein di-Acetate Succinimidyl Ester (CFDA-SE) as a Viability Probe for FACS Analysis.

Volume 7, Number 4: April, 2007 

  • Anaerobic GFP Proteins.
  • Gaussia Luciferase DNA Assays.
  • Ethidium Homodimer (EthD-1)
  • In vitro Luciferin-Galactoside assays.
  • Peptidase Substrates from 6-FMOC-Amino-D-Luciferin.

Volume 7, Number 3: March, 2007 

  • His6-Tag and Fluorescent Nitrilotriacetates (NTAs) for Binding and Measuring Membrane Proteins.
  • Ultrasensitive lacZ Detection in vivo using D-Luciferin-6-O-Galactopyranoside (Luc-Gal).
  • Esterase Assays as a Measure of Cell Viability.
  • Fluorescent Heparin Sulfate Measurement with Berberine.

Volume 7, Number 1: January, 2007 

  • Lysosomal Acid Lipase Deficiency in Wolman’s Disease and Atherosclerosis.
  • Mitochondrial ROS Staining with Dihydroethidium.
  • Cellulase Determination in Arabidopsis thaliana Floral Tissues.
  • New Distributor in Korea.

Volume 6, Number 12: December, 2006 

  •  PET Analysis of Marker Genes.
  •   Photobleaching and Blinking of Fluorophores.
  • EDANS-DABCYL Endopeptidase Substrates.
  • Affordable Value at Marker Gene.

Volume 6, Number 11: November, 2006 

  • Measuring siRNA Gene-Silencing in Mammalian Cells Using Marker Genes.
  • Fluorescent Labeling of Oligonucleotides.
  •  Digoxigenin versus Biotin Labeling.
  • Cellulase Activity Measurements.

Volume 6, Number 10: October, 2006 

  • Measurement of T-Cell Activation using LacZ FACS.
  • Biotin Labeling of Proteins, Nucleotides and Carbohydrates
  • Non-Ionic Detergents for Membrane Biochemistry
  • High-Throughput Microarray Lipase Assay Systems.

Volume 6, Number 9: September, 2006  

  • hGH Reporter Gene Assays
  • Temperature Dependence of Fluorescence.
  • Generic Expression Vectors.
  • Efficient lacZ FACS Analysis in Bacteria.

Volume 6, Number 8: August, 2006 

  • Alkylguanine Transferase (AGT) Fusion.
  • Alkaline Phosphatase Quantitation.
  • Direct Fluorescent Labeling for Determination of Sugars.
  • Apoptosis Detection in Plant Cells.

Volume 6, Number 7: July, 2006 

  • Metastasis Detection using Enzymology.
  • Mitochondrial Enzyme Analysis.
  • New Long-Wavelength Lipase Substrate and Kit.
  • Luciferase Containing Vectors for analysis of Transcription Factors.

Volume 6, Number 6: June, 2006 

  • DNA Transfection Using Dendrimers.
  • Long-Wavelength Phosphatase Analysis.
  • Krabbe’ Disease Lysosomal Glycosidase Assays.
  • Glycoprotein Analyses with a new Long-Wavelength Probe: M1202.

Volume 6, Number 5: May, 2006 

  • Tyrosinase as a Marker Gene in Fungal and Mammalian Cell Lines.
  • Biotin Labeling for Analysis or Purification.
  • Cytochrome P450 Chemiluminescent Assays.
  • Discreet PEG linkers for Biological Applications.
  • Analysis of Lipid Rafts in Cell and Tissue Membranes.

Volume 6, Number 4: April, 2006 

  • Gaussia Luciferase Assays.
  • New Molecular Rotors.
  • New Inhibitor of Lipase and b-Lactamase.
  • Microsomal Dealkylase and Cytochrome P450 Measurement in Live Cells.

Volume 6, Number 3: March, 2006 

  • Lysosomal Storage Disease Detection .
  • Phosphodiesterase Analysis Techniques.
  • Fluorescence of Native GFP and Proteins and Peptides.
  • Alkaline Phosphatase Analysis of Pluripotent Stem Cells.

Volume 6, Number 2: February, 2006 

  • Thymidine Kinase as an in vivo Marker Gene.
  • Artificial Chromosome Expression (ACE) Systems.
  • 4-Methylumbelliferyl Galactoside (MUG).
  • Intron splicing for improved expression of GUS.

Volume 6, Number 1: January, 2006 

  • Pigmentation Gene Localized in MNT1 Cells.
  • FRET for Monitoring of Peptide and Protein Interactions.
  • ROS measurement in mitochondria.
  • New Proline Derivative for Use in Peptidomimetic Studies.

Volume 5, Number 12: December, 2005 

  • Improved Transfection Reagents.
  • Cell / Tissue Permeant D-Luciferin.
  • ROS measurement in live cells.
  • Biotin Labeling for Immunochemistry Applications.
  • Marker Gene Now Accepts Major Credit Cards.

Volume 5, Number 11: November, 2005 

  • Circadian Rhythms Monitored Using an mPer2 Luciferase Construct.
  • Viability Assays for Eukaryotic Cells.
  • New lacZco Vector Systems.
  • New Fluorogenic Substrate for Caspase 3/7.
  • Stock up and Save! Price Increase Effective 1/1/06

Volume 5, Number 10: October, 2005 

  • Vital Stains for lacZ Transfected Cells.
  • Measuring Hypoxia in Tumor Cells.
  • Near Infrared Dyes for Tumor Monitoring.
  • New Fluorogenic Substrate for Caspase 3/7.
  • Fluorescently Labeled Avidin and Streptavidin Assays.

Volume 5, Number 9: September, 2005 

  • Live Plant GUS Assay Systems.
  • Ultrasensitive Homogeneous Immunoassays using D-Luciferin-6-O-Galactopyanoside (Luc-Gal).
  • TMA-DPH, a Fluorescent Probe of Membrane Interiors.
  • Marker Gene Adds Helix Research Fluorescent Reagents.
  • Plant “Florigen” factor identified.

Volume 5, Number 8: August, 2005 

  •   Plant Flowering Mechanisms.
  • Matrix Metalloproteinases Involved in Tumor Invasiveness.
  • Live Cell Yeast Luciferase Assays.
  • Marker Gene Adds Helix Research Fluorescent Reagents.
  • Product of the Month M0545: Dihydrorhodamine 123 (DHR123).

Volume 5, Number 7: July, 2005 

  • Fluorescent X-Gal Substrates.
  • New Dual-Function Fluorescent Glutathione Transferase Assay Kit.
  • Endothelial Lipase Assays.
  • Environment Sensitive Fluorescent Lipid - C12-NBD.

Volume 5, Number 6: June, 2005 

  • Peptidase Assays Using Aminoluciferin Light-Up.
  • Flow cytometry (FACS) lacZ b-Galactosidase Assay.
  • New Protein Mobility Probe.
  • New Cholinesterase Detection Kit.
  • Fluorescent Lysyl Oxidase Assay Systems.

Volume 5, Number 5: May, 2005 

  • Embryonic Stem Cell Gene Trap Methods.
  • Mammalian GUS Marker Gene Systems.
  • Mechanism of Luciferase Action.
  • Chloramphenicol Acetyl Transferase (CAT) Assay Systems.

Volume 5, Number 4: April, 2005 

  • Measuring Gene Regulation Using Marker Genes.
  • Leucine Aminopeptidase as a Measure of Hepatic Function.
  • Cell Surface Engineering for Molecular Recognition.
  • Antifade Reagents for Fluorescence Microscopy and Analysis.
  • Now Order Marker Gene Products through VWR and Fisher Scientific!

Volume 5, Number 3: March, 2005 

  • Genetically Engineered Cell Lines for Detecting Viruses.
  • DNA Quantitation using Fluorescent Dye Staining Assays.
  • Recombinant Arabidopsis Detects Explosives Residue.
  • Routine Coliform Detection using Fluorogenic Substrates.

Volume 5, Number 2: February, 2005 

  •  Leaky RNAi Targeting.
  • Gaucher’s Disease – a Lysosomal Storage Disorder.
  • Barnase Expression Utilizes Negative Selection.
  • Fluorescent Glutathione Transferase Assay.
  • DAAO Selection Marker Technology Available for Licensing.

Volume 5, Number 1: January, 2005 

  • Smallpox Virus to be Labeled with GFP.
  •  Fluorescent Protein Phosphatase Assays.
  • 6-Amino-D-Luciferin Hits the Spotlight.
  • Tetracycline b-D-Galactoside Links Two Vector Systems.
  • DAPBA Detection of Brassinosteroids in Plants.

Volume 4, Number 12: December, 2004 

  • Somatic Hypermutation to Produce New Fluorescent Proteins.
  • aGalA Gene Therapy Localization in Fabry disease.
  • Choosing Optical Filter Sets for Fluorescence Applications.
  • A "Read-Write-Erase" Fluorescent Protein - Dronpa.
  • Live Cell Viral Entry Assay Using Luciferase.

Volume 4, Number 11: November, 2004 

  • Cell Marking with EosF.
  • GUS Activity Measurements in Seeds and Seed Tissues.
  • lacZ Nuclear Localization Vectors.
  • A “Read-Write” Fluorescent Protein – DRONPA.
  • Genetic Analysis using RNAi.

Volume 4, Number 10: October, 2004 

  • Monitoring Transgene Silencing.
  • Bacterial Viability Stains.
  • A New Convertible Green-Red Fluorescent Protein.
  • Fluorescent GUS Detection with DMFDG.
  • NBD-Hydrazide for Fluorescent Carbohydrate Analysis.

Volume 4, Number 9: September, 2004 

  • Improved b-galactosidase detection in lacZ transfected cells.
  • Nonviral Gene Transfer and Targeting in vivo.
  • Mitochondrial Functional Analysis.
  • New GUS Systems for recombinant Plant Analysis.

Volume 4, Number 8: August, 2004 

  • Intracellular GFP Localization using Fusion Proteins.
  • Nanoparticles for in vivo Marker Gene Delivery.
  • New lacZco Vectors Now Available.
  • New Apoptosis Pathways in Plants.
  • Serum Lysophosphatidic Acid (LPA) Assay.

Volume 4, Number 7: July, 2004 

  • New DAAO Positive Selection Marker.
  •   Agrobacterium tumefaciens for Plant Transformations.
  • FACS Blue lacZ b-Galactosidase Detection Kit.
  • A New M. tuberculosis Marker.
  • Lysophosphatidic Acid (LPA) as an Early Diagnostic Tool for Ovarian Cancer.

Volume 4, Number 6: June, 2004 

  • Monitoring mRNA in Living Cells.
  • Mammalian Two-Hybrid Systems.
  • Transfection Efficiency using neo.
  • New Plant Live:Dead Assays.
  • Cell Counts with CFDA-NHS.

Volume 4, Number 5: May, 2004 

  • New Mammalian GFP Vectors.
  • Nanotechnology’s Quantum Dots Now Appearing in Live Cell Applications.
  • New Aza-Epoxide Peptidase Inhibitors for Apoptosis Research.
  • New in vivo GUS Tissue Assays.
  • Waveguide Fluorescent Assay Systems.

Volume 4, Number 4: April, 2004 

  • TAMRA Protein/Antibody Labeling.
  • Retroviral Titer Estimated Using Marker Genes.
  • Toward MRI Imaging of lacZ in vivo.
  • Transgenic versus Endogenous Enzyme Analysis.
  • Depurination and DNA Strand Breakage.

Volume 4, Number 3: March, 2004 

  • Honing in on SARS Detection and Treatment.
  • Cah/Nitrile Hydratase Selection Marker.
  • TAMRA Labeling of DNA, Proteins, and Live Cells.
  • Mutagenicity Testing in Live Plants with GUS.
  • Depurination and DNA Strand Breakage.

Volume 4, Number 2: February, 2004 

  • Bacterial Quantitation Using FDG.
  • lux Marker Gene Used for Tracing Bacterial Growth in vivo.
  • Antibody Labeling with FITC.
  • New lacZ Vector with High Expression Efficiency.
  • New Chemiluminescent lacZ beta-Galactosidase Detection Kit.

Volume 4, Number 1: January, 2004 

  • Gene Silencing Vectors Containing Marker Genes.
  • Senescence Induction Analysis.
  • Removal of Marker Genes by Recombination.
  • Transgenic lacZ Mouse Models.
  • Firefly Luciferase Assays in Tissues.

Volume 3, Number 12: December, 2003 

  • "Fluorobodies" Combine GFP with Antibody Binding.
  • CCPGCC Tetracysteine Reporter Gene.
  • New Chemiluminescent lacZ b-Galactosidase Detection Kit.
  •  New Dehalogenase Reporter Gene.
  • News from the American Society for Cell Biology Meeting in San Francisco.

Volume 3, Number 11: November, 2003 

  • Cell Proliferation Assays Using Labeling Probes.
  • Cell Permeant Caspase/Apoptosis Assays.
  • Neuraminidase Assays Using Fluorescence Detection.
  • Microbial Biosensors Using lacZ Reporting.
  • American Society for Cell Biology Meeting in San Francisco.

Volume 3, Number 10: October, 2003 

  • FLP and Cre Recombinase Induce GFP and lacZ.
  • Ice Nucleation Protein (InaZ) Reporter Gene.
  • Nuclear Compartmentalization Causes Down-Regulation of Marker Genes.
  • High-Efficiency Transfection using "Adenofection."
  • New Renilla, Gaussia, and Pleuromamma Luciferases.

Volume 3, Number 8: August, 2003 

  • Intron Splicing of GUS Used to Track Agrobacterium Plant Transformation.
  • lacZ Two-Hybrid Systems for Tracking Protien Interactions in Live Cells.
  • Neutrophil Function and Chronic Granulomatous Disease Assays.
  • New GUS Kit for Recombinant Plant Analysis.
  • Quenched Peptidase Substrates for Many Applications.

Volume 3, Number 6: June, 2003 

  • New Chemiluminescence Kits.
  • lacZ b-Galactosidase.
  • b-Glucuronidase (GUS).
  • Neuraminidase (5NAcNeu).

Volume 3, Number 5: May, 2003 

  • Apoptosis vs. Necrosis by FACS Analysis.
  • Promoter Analysis Using Marker Genes.
  • Cathepsin Protease Detection Kits.
  • Carbohydrate Analysis/Detection Kits.
  • NPTII Assays in Plants.

Volume 3, Number 4: April, 2003 

  • Combined lacZ and GFP Detection.
  • b-Lactamase as a Marker for Gene Expression.
  • Fungal Identification Using PCR Analysis.
  • Secondary Screening of Blue-White Colonies.
  • New Catalog Now Available!

Volume 3, Number 3: March, 2003 

  • a-Galactosidase Marker Gene for Yeast.
  • New 800 Number for Marker Gene!
  • Toward a Marker Gene for Colorectal Tumors.
  • Hydrophobic Stains for 2D-SDS-PAGE.
  • AHAS Reporter Gene in Plants.

Volume 3, Number 2: February, 2003 

  • Removal of Marker Genes.
  • Small Molecule Activator of Caspase-3.
  • Quantum Dot Fluorescence Applications.
  • Stems Cells found Totipotent by lacZ Staining.
  • New Apoptosis Detection Kits.

Volume 3, Number 1: January, 2003 

  • Higher Transfection Efficiency With Introns.
  • Live Cell Firefly Luciferase Assays.
  • New Proteomics Hydrophobicity Assay for SDS-PAGE Gels.
  • New Plant Selection System Using Mannose.
  • b-Gal and b-GlcU  Chemiluminescent Substrates.

Volume 2, Number 12: December, 2002 

  • Cell Viability/Cytotoxicity Assay
  • Ecdysone-Inducible Promoter Systems for lacZ b-Galactosidase and GFP.
  • Caspase and Serine Protease (Serpase) Activation During Apoptosis.
  • ELISA Detection of b-Gal Using FDG.
  • Suppliers of Reporter Assay Screening Systems.

Volume 2, Number 11: November, 2002 

  • Bacterial Detection using FDG
  • Whole body Luciferase detection in vivo.
  • Protein traffic using “caged-GFP”.
  • Human Antibody Production in Plants.
  • Lipase Activity measured in Live Cells.

Volume 2, Number 10: October, 2002 

  • "Jet-Lag" and Circadian Rhythms in Plants.
  • FDG Staining of live Zebrafish Embryos.
  • Oxidative Burst in Neutrophils with DHR123.
  • Adoptive Immunotherapy for Melanomas.
  • Black Spot Disease in Citrus Fruits.

Volume 2, Number 9: September, 2002 

  • FDG Used for Live Tissue Staining.
  • Multiple Fluorescence Labeling of DNA Probes.
  • Using a Molecular Flashlight for Antiviral Treatment.
  • Marker Gene Adds Daiichi as our New Distributor in Japan.
  • New Strategy against AIDS using recombinant Caspase-3.