Acridine Orange

Product ID: M1851

Unit SizePriceQuantity 
10 mg
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Availability: In stock


Acridine Orange is a lysomotropic probe that can also be used for analysis of autophagy and to label DNA in living cells.

Acridine Orange is a cell-permeant dye that accumulates in acidic organelles in a pH-dependent manner and has been used for autophagy detection as well as for cell cycle determination. It can be used for cell-cycle studies because it emits with a green fluorescence (ex. max: 502 nm; em. max: 525 nm) when bound to dsDNA but with red fluorescence when bound to ssDNA or RNA (ex. max: 460 nm; em. max: 650 nm). The same red/green shift has also been used for lysosomal analysis in fluorescence microscopy and flow cytometric analysis since it emits green at neutral pH but with red fluorescence in acidic environments. It has also found use in cellular physiology studies and in the fluorescent microscopic examination of microorganisms. Acidotropic dyes like Acridine Orange will typically only stain late autophagic vacuoles.

Technical Data
SKU M1851
CAS Number 10127-02-3
Unit Size 10 mg
Alternative Names 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
Detection Method Fluorescence
Molecular Weight 369.96
Soluble In H2O, DMSO
Storage Conditions 22-25C, Protect from Light

References and Citations


  • Mozhenok TP, Beliaeva TN, Bulychev AG, Kuznetsova IM, Leont'eva EA, Faddeeva MD (1997) "The effect of biologically active compounds on lysosome fusion with phagosomes and the F-actin content in mouse peritoneal macrophages and on the status of the lysosomal membranes in mouse hepatocytes." Tsitologiia Journal 39:552-559.
  • Horn W, Hansmann C, Federlin K (1985) "An improved fluorochrome microassay for the detection of living and non-living intracellular bacteria in human neutrophils." J. Immunol. Methods (1985) 83:233-240.
  • Wissing F, Sanchez CP, Rohrbach P, Ricken S, Lanzer M, (2002) "Illumination of the malaria parasite Plasmodium falciparum alters intracellular pH. Implications for live cell imaging. J. Biol. Chem. 277:37747-37755.
  • Patschan S, Chen J, Gealekman O, Krupincza K, Wang M, Shu L, Shayman JA, Goligorsky MS, (2008) "Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells." Amer. J. Physiol. Renal Physiol 294:F100-F109.
  • Darzynkiewicz Z (1990) "Differential staining of DNA and RNA in intact cells and isolated cell nuclei with acridine orange. Methods Cell Biol. 33:285-298.
  • Barasch J, Kiss B, Prince A, Saiman L, Gruenert D, al-Awqati Q (1991) "Defective acidification of intracellular organelles in cystic fibrosis." Nature 352:70-73.
  • Kågedal K, Zhao M, Svensson I, Brunk UT (2001) "Sphingosine-induced apoptosis is dependent on lysosomal proteases. Biochem. J. 359:335-343.
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