AlisaRed™

Product ID: M1787



Unit SizePriceQuantity 
1 mg
$29.37
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Description

AlisaRed™ is a very useful redox substrate that reacts with hydrogen peroxide to produce the highly fluorescent dye resorufin with red emission (585 nm). The AlisaRed™ system can be coupled with other enzymes for ultrasensitive quantitation of a diverse assortment of analytes.

AlisaRed™ is Marker Gene's high purity Amplex Red® equivalent. Also known as N-Acetyl-3,7-dihydroxyphenoxazine (ADHP), it is a highly sensitive, stable peroxidase fluorogenic substrate used as a hydrogen peroxide probe. It can also be coupled with horseradish peroxidase (HRP) to detect other analytes. AlisaRed™ is a colorless and non-fluorescent reagent that reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, AlisaRed™ can detect as little as 5 pmol of H2O2 in a 100 µL sample/well. The AlisaRed™ system has been coupled with many other enzymes for ultrasensitive quantitation of a diverse assortment of analytes, including glucose (using glucose oxidase), galactose (using galactose oxidase), cholesterol (using cholesterol esterase and cholesterol oxidase), glutamic acid (using glutamate oxidase), xanthine or hypoxanthine (employing xanthine oxidase), uric acid (employing uricase), choline and acetylcholine (acetylcholine esterase), inorganic phosphate and pyrophosphate (coupling maltose phosphorylase and glucose oxidase).

Technical Data
SKU M1787
CAS Number 119171-73-2
Unit Size 1 mg
Alternative Names 1-(3,7-Dihydroxyphenoxazin-10-yl)ethanone, ADHP, Amplex Red®, 10-​Acetyl-​3,7-​dihydroxyphenoxazine
Absorption 560 nm
Emission Wavelength 585 nm
Detection Method Fluorescence
Molecular Formula C14H11NO4
Molecular Weight 257.24
Soluble In DMSO
Storage Conditions -20C, Protect From Light, Desiccated

References and Citations

Citations:

  • Harlan FK, Lusk JS, Mohr BM, Guzikowski AP, Batchelor RH, Jiang Y, et al. (2016) "Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities." PLoS ONE 11(5): e0156312.
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    References:

    • Debski D, Smulik R, Zielonka, J, Michalowski B, Jakubowska M, Debowska K, Adamus, J Marcinek, A, Kalyanaraman B, Sikora, A, (2016) "Mechanism of oxidative conversion of Amplex Red to resorufin: Pulse radiolysis and enzymatic studies." Free Radical Biology & Medicine 95: 323-332.
    • Miwa S, Treumann A, Bell A, Vistoli G, Nelson G, Hay S, von Zglinicki T, (2016) "Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays." Free Radical Biology & Medicine 90: 173-183.
    • He X, Chen F, McGovern MM Schuchman EH (2002) "A fluorescence-​based, high-​throughput sphingomyelin assay for the analysis of Niemann-​Pick disease and other disorders of sphingomyelin metabolism." Analytical Biochemistry 306(1): 115-23.
    • Fortelius M, Mattjus P, (2006) "Galactose oxidase action on galactose containing glycolipids-​-​a fluorescence method." Chemistry and physics of lipids142(1-2): 103-110.
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