MarkerGene™ BCA Protein Assay Kit

Product ID: M2533



Unit SizePriceQuantity 
1 kit
$92.40
  • Buy 5 for $73.92 each and save 20%

Availability: In stock


Description

This kit employs a modification of the bicinchoninic acid (BCA) method for the colorimetric detection of protein concentration or total protein determination in a sample.

This protocol details a method for a high range assay when the protein in the sample is expected to be between 125 and 2000 µg/mL and a high/low range assay for a wider variety of samples where the protein concentration is expected to be between 5 and 2000 µg/mL. Also detailed in this protocol is a method of measuring the total amount of protein in cell lysates in order to normalize protein levels for coupled assays where cell number or total protein is of interest. It is important to note that because the BCA reaction with protein does not reach a true end point, color development continues the longer a sample is left to incubate. However, using the incubation method detailed in this protocol, the color development is significantly slowed, allowing for multiple samples to be assayed simultaneously.

Technical Data
SKU M2533
Unit Size 1 kit
Alternative Names Smith Assay Kit
Detection Method Colorimetric
Storage Conditions See individual vials for storage conditions

References and Citations

References:

  • Krieg RC, Dong Y, Schwamborn K, Knuechel R. (2005) “Protein quantification and its tolerance for different interfering reagents using the BCA-method with regard to 2D SDS PAGE.” J. Biochem. Biophys. Methods 65, 13-19.
  • Brown RE, Jarvis KL, Hyland KJ. (1989) “Protein measurement using bicinchoninic acid: Elimination of interfering substances.” Anal. Biochem 180, 136-139.
  • Wiechelman KJ, Braun RD, Fitzpatrick JD. (1988) “Investigaton of the bicinchoninic acid protein assay: Identification of the groups responsible for color formation.” Anal. Biochem 175, 231-237.
  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. (1985) “Measurement of protein using bicinchoninic acid.” Anal. Biochem 150, 76-85.
  • Lowry OH, Rosebrough NJ, Farr LA, Randall RJ. (1951) “Protein measurement with the folin phenol reagent.” J. Biol. Chem 193, 265-275.
>Show more
>Show less

Click here for Newsletter articles about this product



Technical Support

Question about this product? Ask a Scientist!

We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:


There are currently no frequently asked questions for this product, click the button above to ask a question

Recently Viewed Products