Beta-Galactosidase

β-Galactosidase, an important reporter gene marker encoded by lacZ, is commonly used for monitoring transfection efficiency in mammalian, yeast, and bacterial cells and identifying expression of recombinant fusion genes. E. coli β-galactosidase has a high turnover rate, hydrolyzing D-galactose from various β-galactosides, which allows for very low level detection in common cell types. β-Galactosidase substrates can also be used in conjunction with galactosidase-conjugated secondary detection agents in enzyme linked immunosorbent assays (ELISA) formats and in immunohistochemical techniques.

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  1. Fluorescein mono-β-D-galactopyranoside

    Fluorescein mono-β-D-galactopyranoside

    $164.65

    Convenient β-galactosidase fluorescent probe. Especially useful for enzymatic analyses where 1st order kinetics are desired. Learn More
  2. Chloramphenicol β-D-galactoside

    Chloramphenicol β-D-galactoside

    $131.65

    Upon enzymatic or chemical hydrolysis of the galactoside group, chloramphenicol, an antibiotic (bacteriostatic), is produced. Learn More
  3. 5-Fluorouridine 5'-O-β-D-galactopyranoside

    5-Fluorouridine 5'-O-β-D-galactopyranoside

    $131.65

    Analog of 5-fluorouridine (5-FUR) useful for lacZ specific release of 5-FUR in recombinant cells or tissues. Learn More
  4. Tetracycline 10-O-β-D-galactopyranoside

    Tetracycline 10-O-β-D-galactopyranoside

    $131.65

    This tetracycline analog useful as an antibiotic with specific release in lacZ transfected cells and tissues. Learn More
  5. Resorufin β-D-galactopyranoside

    Resorufin β-D-galactopyranoside

    $56.75

    Red fluorogenic substrate for lacZ β-galactosidase activity, intracellularly, or in vitro. Learn More
  6. 4-Methylumbelliferyl β-D-galactopyranoside (MUG)

    4-Methylumbelliferyl β-D-galactopyranoside (MUG)

    $65.43

    Bright blue fluorogenic β-galactosidase substrate, especially useful for in vitro assays of cloned lacZ expression. Learn More
  7. Fluorescein di-β-D-galactopyranoside (FDG)

    Fluorescein di-β-D-galactopyranoside (FDG)

    $132.40

    Ultra-sensitive substrate for lacZ β-galactosidase detection in vivo and in vitro; useful in one of the most common transfection optimization assays in molecular biology. Learn More
  8. 4-Trifluoromethylumbelliferyl-β-D-galactopyranoside (TFMU-Gal)

    4-Trifluoromethylumbelliferyl-β-D-galactopyranoside (TFMU-Gal)

    $131.65

    Long wavelength, blue-green fluorogenic β-galactosidase probe that has a lower pKa for higher fluorescence in the physiological pH range. Learn More

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