MarkerGene™ β-Galactosidase Staining Kit

Product ID: M1352



Unit SizePriceQuantity 
1kit
$177.72
  • Buy 5 for $142.18 each and save 21%

Availability: In stock


Description

Can be used to monitor and detect enzyme activity in stably or transiently transfected cells or transgenic tissues using the sensitive chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal).

The E. coli lacZ gene is among the most widely used marker genes for mammalian, yeast and bacterial transfection studies due in part to its resistance to intracellular proteolytic degradation. The lacZ gene encodes for the β-Galactosidase (β-Gal) enzyme that catalyzes the hydrolysis of a wide range of β-galactosides. When β-Gal cleaves the glycosidic linkage in X-Gal, a soluble, colorless indoxyl derivative is produced which quickly dimerizes and becomes oxidized to produce a bright blue indigo dye precipitate at the site of β-Gal activity. The dimerization and oxidation reaction is facilitated, in this kit, by a developer buffer solution containing ferric and ferrous ions. Both transfection efficiency and comparative expression levels can be determined by examining and counting the number of blue cells in the total cell population.

Technical Data
SKU M1352
Unit Size 1kit
Absorption nm
Detection Method Colorimetric
Notes The kit contains enough reagents and buffers for up to 100 assays, when run in a microplate assay format, and also includes a detailed protocol for use in detection of β-galactosidase in mammalian cells and tissues.

References and Citations

Citations:

  • Hägerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams RH, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F. (2013) "A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy." EMBO J. 32(5):629-44.
  • Surdenikova L, Ru F, Nassenstein C, Tatar M, Kollarik M. (2012) "The neural crest- and placodes-derived afferent innervation of the mouse esophagus." Neurogastroenterol Motil. 24(10):e517-25
  • Nassenstein C, Taylor-Clark TE, Myers AC, Ru F, Nandigama R, Bettner W, Undem BJ. (2010) "Phenotypic distinctions between neural crest and placodal derived vagal C-fibres in mouse lungs." J Physiol. 588(Pt 23):4769-83.
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References:

  • Bout A, Valerio D, Scholte BJ (1993) "In vivo transfer and expression of the lacZ gene in the mouse lung." Exp. Lung Res. 19(2): 193-202
  • Kobayashi S, Okada M (1993) "A double staining technique using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and immunoperoxidase in whole Drosophila embryos." Biotech. Histochem. 68(4): 237-9.
  • Kruger A, Schirrmacher V, von Hoegen P (1994) Scattered micrometastases visualized at the single-cell level: detection and re-isolation of lacZ-labeled metastasized lymphoma cells." Intl. J. Cancer) 58(2): 275-84.
  • Singh MB, Knox RB (1984) "Quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method." Histochem 16(12): 1273-96.
  • Kadokawa Y, Suemori H, Nakatsuji N (1990) "Cell lineage analyses of epithelia and blood vessels in chimeric mouse embryos by use of an embryonic stem cell line expressing the beta-galactosidase gene." Cell Differ. Develop.29(3): 187-94.
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