The β-glucuronidase (GUS) enzyme from E. coli has been well documented to provide desirable characteristics as a marker gene in transformed plants.
The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport.
Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule. This allows for high sensitivity fluorometric, and spectrophotometric measurements of β-glucuronidase gene fusion expression.
This high copy number plant vector, pNosdcGUS, expresses the GUS gene under the control of the nopaline synthase (pNos) promoter. This vector may be used to monitor virus production and transfection efficiency. Learn More