Beta-Glucuronidase (GUS)

The β-glucuronidase (GUS) enzyme from E. coli has been well documented to provide desirable characteristics as a marker gene in transformed plants.

The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport.

Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule. This allows for  high sensitivity fluorometric, and spectrophotometric measurements of β-glucuronidase gene fusion expression.

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  1. 4-Methylumbelliferyl β-D-glucuronide (MUGlcU)

    4-Methylumbelliferyl β-D-glucuronide (MUGlcU)

    $44.86

    Common blue fluorogenic GUS substrate for measuring cloned activity in plant lysate, homogenate, leaf disk or whole plant assays. Learn More
  2. 6-Chloro-indolyl β-D-glucuronic acid, lithium salt

    6-Chloro-indolyl β-D-glucuronic acid, lithium salt

    $83.39

    Chromogenic substrate for β-D-Glucuronidase(GUS). Learn More
  3. Carboxyumbelliferyl β-D-glucuronide (CUGlcU)

    Carboxyumbelliferyl β-D-glucuronide (CUGlcU)

    $98.80

    Water soluble fluorogenic GUS substrate similar to MUGlcU. Learn More
  4. Bromoxynil glucuronic acid methyl ester

    Bromoxynil glucuronic acid methyl ester

    $95.40

    Bromoxynil derivative which can be used to selectively ablate GUS-positive cells and tissues in plant systems. The methyl ester helps with cell permeation in vivo. Learn More
  5. MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit

    MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit

    $298.88

    Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate. Learn More
  6. Fluorescein di-β-D-glucuronide, dimethyl ester

    Fluorescein di-β-D-glucuronide, dimethyl ester

    $131.65

    A cell-permeant β-glucuronidase substrate for live cell and tissue assays of cloned GUS marker gene activity and transfection levels. Learn More
  7. pNosdcGUS Plant GUS Expression Vector

    pNosdcGUS Plant GUS Expression Vector

    $310.20

    This high copy number plant vector, pNosdcGUS, expresses the GUS gene under the control of the nopaline synthase (pNos) promoter. This vector may be used to monitor virus production and transfection efficiency. Learn More
  8. EDANS-GalNAc

    EDANS-GalNAc

    $51.05

    Reference standard for use with the MarkerGene™ Carbohydrate Analysis Detection Kit M0272. Learn More

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