The β-glucuronidase (GUS) enzyme from E. coli has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport. Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule. This allows for histochemical, fluorometric, and spectrophotometric measurements of β-glucuronidase gene fusion expression.
This kit allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity (live cells) with a blue emitting dye while staining of nucleic acids in chromatin (dead cells) with a green emitting dye. Learn More