Beta-Glucuronidase

The β-glucuronidase (GUS) enzyme from E. coli has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. The enzyme is also capable of tolerating amino-terminal additions, making it useful for study of plant organelle transport. Various β-glucuronic acid substrates are available for detection of GUS expression, all of which contain the sugar D-glucopyranosiduronic acid attached by glycosidic linkage to a hydroxyl group of a chromogenic, fluorogenic, or other detectable molecule. This allows for histochemical, fluorometric, and spectrophotometric measurements of β-glucuronidase gene fusion expression.

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  1. 4-Methylumbelliferyl β-D-glucuronide (MUGlcU)

    4-Methylumbelliferyl β-D-glucuronide (MUGlcU)

    $44.86

    Common blue fluorogenic GUS substrate for measuring cloned activity in plant lysate, homogenate, leaf disk or whole plant assays. Learn More
  2. 6-Chloro-indolyl β-D-glucuronic acid, lithium salt

    6-Chloro-indolyl β-D-glucuronic acid, lithium salt

    $83.39

    Chromogenic substrate for β-D-Glucuronidase(GUS). Learn More
  3. Carboxyumbelliferyl β-D-glucuronide (CUGlcU)

    Carboxyumbelliferyl β-D-glucuronide (CUGlcU)

    $98.80

    Water soluble fluorogenic GUS substrate similar to MUGlcU. Learn More
  4. MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit

    MarkerGene™ β-Glucuronidase (GUS) Reporter Gene Activity Detection Kit

    $298.88

    Allows for quantitative measurement of GUS enzyme activity in transformed plants through use of a fluorogenic substrate. Learn More
  5. Fluorescein di-β-D-glucuronide, dimethyl ester

    Fluorescein di-β-D-glucuronide, dimethyl ester

    $131.65

    A cell-permeant β-glucuronidase substrate for live cell and tissue assays of cloned GUS marker gene activity and transfection levels. Learn More
  6. MarkerGene™ Live:Dead/Cytotoxicity Assay Kit, Blue/Green Staining

    MarkerGene™ Live:Dead/Cytotoxicity Assay Kit, Blue/Green Staining

    $247.39

    This kit allows fast and easy measurement of both living and dead cells by measuring intracellular esterase activity (live cells) with a blue emitting dye while staining of nucleic acids in chromatin (dead cells) with a green emitting dye. Learn More

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