MarkerGene™ Biotin Detection and Quantitation Kit
Product ID: M1356
Allows the measurement of the molar ratio of biotin to protein in biotin-protein conjugates, biotin-DNA molar ratios in biotinylated DNA samples, or quantitation of biotin concentrations in solution.
|Notes||The kit can be used to determine biotin concentrations down to 1 uM concentrations. Includes enough reagents for 100 assays using a microtiterplate format (96-well plate, 200uL assay volume) or 20 assays using a spectrophotometer (1mL assay volume).|
- Batchelor RH, Sarkez A, Cox WG, Johnson I (2007) "Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids." Biotechniques 43(4): 503-7.
- Ikariyama Y, Aizawa M (1988) "Bioaffinity sensors." Methods Enzymol. 137: 111-24.
- Livnah O, Bayer EA, Wilchek M, Sussman JL (1993) "The structure of the complex between avidin and the dye, 2-(4'-hydroxyazobenzene) benzoic acid (HABA)." FEBS Lett. 328(1-2): 165-8.
Question about this product? Ask a Scientist!
We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:
There are currently no frequently asked questions for this product, click the button above to ask a question