Biotin-dextran (70,000 MW)

Product ID: M0788



Unit SizePriceQuantity 
10mg
$82.30
  • Buy 5 for $65.85 each and save 20%

Availability: In stock


Description

Membrane impermeant dextran conjugate containing covalently attached biotin molecules (Vitamin H) making it useful in streptavidin/avidin assay systems.

This membrane impermeant dextran conjugate can be loaded into cells using either microinjection or by hypoosmotic shock. The attached biotins are commonly used haptens that can be detected by using its strong affinity for the proteins avidin (from egg white) or streptavidin. This conjugate contains approximately 12 biotin groups/dextran (based on amine assay; with approx. 1 amine/40 glucose residues). The excess amino groups are not capped, and they can be further labeled with a fluorophore, or can be fixed in place with formaldehyde, lysine or glutaraldehyde, allowing subsequent tissue processing, such as sectioning, if desired. Note that dextrans of molecular weight less than 100K, can be taken up by plant cells through endocytosis, and thus this derivative can be used to monitor such internalization events. See references below for further information on the use of this standard biotin conjugate.

Technical Data
SKU M0788
Unit Size 10mg
Molecular Weight 73400
Soluble In H2O, DMSO
Storage Conditions 2-8C, Desiccated
Notes White solid

References and Citations

Citations:

  • Wu F, Wu Y, Niu Z, Vollmer F. (2016) "Integrating a DNA Strand Displacement Reaction with a Whispering Gallery Mode Sensor for Label-Free Mercury (II) Ion Detection." Sensors (Basel). 16(8).
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References:

  • Varga C., Sík A., Lavallée P., Deschênes M., "Dendroarchitecture of relay cells in thalamic barreloids: a substrate for cross-whisker modulation." J. Neurosci (2002)22(14): 6186-94.
  • Kurzen H., Manns S., Dandekar G., Schmidt T., Prätzel S., Kräling B.M., "Tightening of endothelial cell contacts: a physiologic response to cocultures with smooth-muscle-like 10T1/2 cells." J. Invest. Dermatol. (2002)119(1): 143-53.
  • Jürgens U., Alipour M., "A comparative study on the cortico-hypoglossal connections in primates, using biotin dextranamine."Neurosci. Lett. (2002)328(3): 245-8.
  • Akkoyun A., Bilitewski U., "Optimisation of glass surfaces for optical immunosensors." Biosens. Bioelectron.(2002)17(8) 655-64.
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