MarkerGene™ Biotin-X Antibody/Protein Labeling Kit

Product ID: M1138



Unit SizePriceQuantity 
1kit
$286.94
  • Buy 5 for $229.55 each and save 20%

Availability: In stock


Description

This kit is designed for efficient biotinylation of antibodies and other proteins and analysis after conjugation.

This kit contains the reactive probe biotin-X succinimidyl ester, which reacts with primary amines of proteins, antibodies or other biomolecules to form stable biotin conjugates. The long spacer (X) between the biotin and the reactive group in biotin-X succinimidyl ester enhances the ability of the conjugated biotin to interact with avidin and streptavidin. Also included in the kit are a gel filtration spin-column to purify the biotinylated protein from any excess biotin as well as detection reagents for quantitating the degree of biotinylation and a control. This kit contains sufficient reagents for at least five biotinylation reactions.

Technical Data
SKU M1138
Unit Size 1kit

References and Citations

References:

  • Perrier AL, Massoulie J, Krejci E., (2002) "PRiMA: the membrane anchor of acetylcholinesterase in the brain." Neuron. 33: 275-285.

  • Peng HB, Xie H, Rossi SG, Rotundo RL., (1999) "Acetylcholinesterase clustering at the neuromuscular junction involves perlecan and dystroglycan." J Cell Biol. 145: 911-921.

  • Haugland, R. P., and Bhalgat, M. K. (1998) Preparation of avidin conjugates. Methods Mol. Biol. 80: 185-196.

  • Haugland, R. P., and You, W. W. (1998) Coupling of antibodies with biotin. Methods Mol. Biol. 80: 173-183.

  • Haugland RP; You WW (1995) Coupling of monoclonal antibodies with biotin. Methods Mol Biol. 45: 223-233.

  • Green, N.M. (1970). Spectrophotometric Determination of Avidin and Biotin. Methods in Enzymology. 18: 418-425.

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