Product ID: M1547

Unit SizePriceQuantity 
100 uL (4mM in DMSO)
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Calcein AM is useful for determining cell viability. In live cells the non-fluorescent calcein AM is hydrolyzed by intracellular esterases into the green fluorescent anion calcein.

Calcein AM is a cell-permeant probe that is widely used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein after acetoxymethyl ester hydrolysis by intracellular esterases. NOTE: Supplied as a convenient * 4mM soln in DMSO

Technical Data
SKU M1547
CAS Number 148504-34-1
Unit Size 100 uL (4mM in DMSO)
Alternative Names Calcein acetoxymethyl ester
Detection Method Fluorescence
Molecular Weight 994.9
Storage Conditions -20C, Protect From Light

References and Citations


  • Kajta M, Wójtowicz AK, Mackowiak M, Lason W. (2009) "Aryl hydrocarbon receptor-mediated apoptosis of neuronal cells: a possible interaction with estrogen receptor signaling." Neuroscience 158(2) 811-22.
  • Fonseca PC, Nihei OK, Savino W, Spray DC, Alves LA. (2006)"Flow cytometry analysis of gap junction-mediated cell-cell communication: advantages and pitfalls." Cytometry A. 69(6) 487-93.
  • Coder DM. (2001) "Assessment of cell viability." Curr Protoc Cytom. Chapter 9:Unit 9.2.
  • Gatti R, Belletti S, Orlandini G, Bussolati O, Dall'Asta V, Gazzola GC. (1998) "Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy." J Histochem Cytochem. 46(8) 895-900.
  • Weston SA, Parish CR. (1990) "New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy." J Immunol Methods. 133(1)87-97.
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