Calcein Blue AM acetate

Product ID: M1791



Unit SizePriceQuantity 
1 mg
$99.00
  • Buy 5 for $79.20 each and save 20%

Availability: In stock


Description

Calcein Blue AM acetate is a cell-permeant, blue fluorescent viability probe. It can be used with other probes to quickly and easily measure the total number of live versus dead cells. It's blue emission won't overlap with other probes having green fluorescence, like GFP.

Calcein Blue AM acetate is a new probe, similar to the green-emitting Calcein AM (Product M1547), that measures both viability and cell-membrane integrity, but with emission in the blue wavelength region (EX 360 nm / EM 450 nm) upon cell uptake and turnover. Calcein Blue AM acetate is virtually non-fluorescent and penetrates live cell membranes, but upon ubiquitous esterase enzyme activity in the cell, a bright blue fluorescent product is formed that is trapped in live cells, making measurement by epifluorescence light microscopy or flow cytometry convenient. It is especially useful when the green channel is already being used for another probe, such as an FITC label antibody or a GFP-based probe, for example. In dead cells, because the membrane is compromised, the dye leaks out quickly and there is no staining. Co-staining dead cells can give a quantitative number for live vs. dead cells in a specific cell culture. And since Calcein Blue AM acetate is non-toxic, it can be incorporated into high-content or high-throughput analysis formats or can be used to measure toxicity of added compounds or conditions.

Technical Data
SKU M1791
Unit Size 1 mg
Emission Wavelength 450 (in cell)
Detection Method Fluorescence
Molecular Formula C23H25NO12
Molecular Weight 507.44
Soluble In DMSO
Storage Conditions -20C, Protect From Light


References and Citations

Citations:

  • Harlan FK, Lusk JS, Mohr BM, Guzikowski AP, Batchelor RH, Jiang Y, et al. (2016) "Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities." PLoS ONE 11(5): e0156312.
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    References:

    • Daniel, JA, Malladi, CS, Kettle E, McCluskey A, Robinson PJ (2012), "Analysis of synaptic vesicle endocytosis in synaptosomes by high-​content screening." Nature Protocols 7(8): 1439-1455.
    • Fuller E, Streger, SH, Rothmel RK, Mailloux, BJ, Hall JA, Onstott TC, Fredrickson JK, Balkwill DL, DeFlaun MF. (2000) "Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments." Applied and Environmental Microbiology 66(10): 4486-4496.
    • Abbaci M, Barberi-Heyob M, Blondel W, Guillemin F, Didelon J, (2008) "Advantages and limitations of commonly used methods to assay the molecular permeability of gap junctional intercellular communication." Biotechniques 45:33-52.
    • Morley N, Rapp A, Dittmar H, Salter L, Gould D, Greulich KO, Curnow A, (2006) "UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells." Mutagenesis 21:105-114.
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