Casein-FITC conjugate

Product ID: M1315

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A conjugated protein substrate used for fluorometric measurement of protease activity in vitro or for use in endocytosis labeling and analysis.

Casein is a naturally occurring protein in milk that is suitable as a general substrate for a myriad of proteases. Labeled with multiple fluorescent dyes, Calein-FITC conjugate exhibits significant fluorescence quenching. Protease-catalyzed hydrolysis releases highly fluorescent-labeled peptides; the accompanying increase in fluorescence is proportional to protease activity and can be conveniently measured in a continuous assay format using a fluorometer equipped with an appropriate (fluorescein) filter set (EX/EM= 490/520 nm).

Technical Data
SKU M1315
Unit Size 5mg
Absorption 480
Emission Wavelength 520
Detection Method Fluorescence
Storage Conditions F, D

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References and Citations


  • Narayanan A, Ridilla M, Yernool DA. (2011) "Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions." Protein Sci. 20(1):51-61.
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  • Homer KA, Beighton D. (1990) "Fluorometric determination of bacterial protease activity using fluorescein isothiocyanate-labeled proteins as substrates." Anal Biochem. 191(1):133–137.
  • Anson ML. (1938) "The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin" J. Gen. Physiol. 22: 79-89.
  • Severini A, Morgan AR. (1991) "An assay for proteinases and their inhibitors based on DNA/ethidium bromide fluorescence." Anal. Biochem. 193: 83.
  • Folin O, Ciocalteu V. (1929) "On tyrosine and tryptophane determinations in proteins." J. Biol. Chem. 73, 627.
  • Twining SS. (1984) "Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes." Anal. Biochem. 143: 30-34.
  • Finehout EJ, Cantor JR, Lee KH, (2005) “Kinetic characterization of sequencing grade modified trypsin” Proteomics 5: 2319-2321.
  • Voss EW, Workman CJ, Mummert ME. (1996) "Detection of protease activity using a fluorescence-enhancment globular substrate" BioTechniques 20(2): 286-291.
  • Bolger R, Checovich W. (1994) "A new protease activity assay using fluorescence polarization" BioTechniques 17(3): 585-589.
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