MarkerGene™ Flow Cytometry Cell Proliferation Analysis Kit
Product ID: M2709
This kit is used to label cells for long-term analysis of proliferation and allows the tracing of cells within cultures.
The ability to monitor cell migration, cell proliferation or to identify cells is important in numerous biological and clinical applications. Fluorescent dyes that emit at wavelengths outside of natural background fluorescence wavelengths are particularly useful for such analyses. The probe CFDA-SE which can passively diffuse into living cells, is colorless and nonfluorescent until its acetate groups are cleaved by intracellular esterase enzyme activity to produce a highly fluorescent, amine-reactive carboxyfluorescein derivative with excitation and emission spectral wavelengths at 488nm and 517 nm respectively. The succinimidyl ester group of CFDA-SE reacts with intracellular amines, forming fluorescent conjugates that are well-retained and can even be fixed using standard glutaraldehyde or paraformaldehyde based fixatives. Excess unconjugated reagent and by-products passively diffuse into the extracellular medium, where they can be easily washed away. The dye–protein adducts that form in labeled cells have been shown to be retained in cells throughout development, meiosis, or even in vivo. In addition, the fluorescence is inherited by daughter cells after cell division or cell fusion, and is not transferred to adjacent cells.
This kit provides CFDA-SE in easy to use, single use vials, allowing for convenient labeling of small volumes of sample without the need to waste reagents.
|Unit Size||1 kit|
|Storage Conditions||-20°C, desiccated and protected from light|
- Weston SA, Parish CR (1990) "New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy." J. Immunol. Methods 133:87-97.
- Karrer FM, Reitz BL, Hao L, Lafferty KJ (1992) "Fluorescein labeling of murine hepatocytes for identification after intrahepatic transplantation." Transplant Proc. 24: 2820-2821.
- Current Protocols in Cytometry , J. P. Robinson, Ed., (1998) pp 9.11.1-9.11.9.
Question about this product? Ask a Scientist!
We pride ourselves on the high quality of our products and want you to get the best possible results from your assays. If you have any questions about this product or need help optimizing your protocol check out the product FAQs below or ask your own question and one of our expert scientists will get back to you asap:
There are currently no frequently asked questions for this product, click the button above to ask a question