5(6)-Carboxyfluorescein diacetate (CFDA)

Product ID: M0011

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  • Buy 5 for $112.19 each and save 21%

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Popular, ultrasensitive, fluorescent substrate for measuring esterase activity in live cells often used in cell viability assays and "Live-Dead" assay systems, especially in flow cytometry.

This reagent is useful for automated cell viability assays because the cleavage product produced upon enzymatic or chemical hydrolysis of the acetate group(s) is carboxyfluorescein Product M0122. This fluorophore is well-retained inside live cells, while the substrate CFDA is membrane permeant. See the references for additional information about CFDA use in bacterial, plant, yeast and mammalian cell culture.   Green emission spectral properties (Abs 488 nm/EM 517 nm) after removal of acetate esters, intracellularly.

Technical Data
SKU M0011
CAS Number 124387-19-5
Unit Size 100mg
Detection Method Fluorescence
Molecular Formula C₂₅H₁₆O₉
Molecular Weight 460.4
Soluble In DMSO
Storage Conditions Frozen, Desiccated
Notes High Purity-Molecular Biology Grade

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References and Citations


  • Mathew M, Tan E, Labonte JW, Shah S, Saeui CT, Liu L, Bhattacharya R, Bovonratwet P, Gray JJ, Yarema K. (2017) "GLYCOENGINEERING OF ESTERASE ACTIVITY THROUGH METABOLIC FLUX-BASED MODULATION OF SIALIC ACID." Chembiochem.
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  • "A trace amount of the human pro-apoptotic factor bax induces bacterial death accompanied by damage of DNA." Asoh S, Nishimaki K, Nanbu-Wakao R., Ohta S. J Biol. Chem. 273(18): 11384-91 (1998).
  • "Subcellular compartmentation of different lipophilic fluorescein derivatives in maize root epidermal cells." Brauer D., et al. Protoplasma 192: 70 (1996).
  • "Role of three Rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast." Singer-Kruger B, Stenmark H, Dusterhoft A, Philippsen P, Yoo JS, Gallwitz D, Zerial M. J Cell. Biol. 125(2): 283-298 (1994).
  • "Measurement of intracellular fluorescence of human monocytes relative to oxidative metabolism." Robinson JP, Bruner LH, Bassoe CF, Hudson JL, Ward PA, Phan SH. J Leukocyte Biol. 43(4): 304-310 (1988).
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