Chloramphenicol Acetyltransferase (CAT) catalyzes the transfer of an acetyl group from acetyl CoA to chloramphenicol. Being a bacterial gene, CAT can be expressed in mammalian cells and assayed for with no background activity. CAT is traditionally assayed for using thin layer chromatography, though fluorescent substrates are also commercially available. Marker Gene provides a variety of high copy number expression vectors and is constantly developing new vectors and systems. Feel free to call our technical assistance staff for more information about these or other molecular biology products for your research.
High copy number eukaryotic vector, pCdgCAT expresses the E. coli Chloramphenicol Acetyltransferase (CAT) gene under the control of the cytomegalovirus immediate early gene (CMV IE) promoter. Very useful for transfection of mammalian cells in culture and for use in other species. Learn More
Calcein Blue AM acetate is a cell-permeant, blue fluorescent viability probe. It can be used with other probes to quickly and easily measure the total number of live versus dead cells. It's blue emission won't overlap with other probes having green fluorescence, like GFP. Learn More