Coelenterazine (native form)

Product ID: M0739



Unit SizePriceQuantity 
50uL
$54.43
  • Buy 5 for $43.54 each and save 21%

Availability: In stock


Description

Coelenterazine is the native form of the luminescent enzyme substrate for apoaequorin, gaussia and renilla luciferases. This substrate is also a component of our convenient assay kit, MarkerGene™ Gaussia Luciferase Cellular Assay Kit, Product M1193, for quickly measuring the luciferase activity in mammalian cell culture.

The coelenterazine/luciferase systems from Renilla, Gaussia, Aequorea and Watesenia luciferases are widely used as marker genes in a variety of cell types. This substrate, coelenterazine, is common to many of the marine bioluminescent reactions, where it is either utilized as a simple substrate, being catalytically turned over in the bioluminescent reaction, or in others, such as in the photoprotein systems of Mneiopsis, where it is incorporated as part of the photoprotein. The gaussia and renilla luciferase systems have been used as a very sensitive indicator of intracellular calcium ion concentrations, superoxide anion and peroxynitrite in cells or tissues.

Technical Data
SKU M0739
CAS Number 55779-48-1
Unit Size 50uL
Emission Wavelength 470
Detection Method Fluorescence
Molecular Formula C₂₆H₂₁N₃O₃
Molecular Weight 423.46
Soluble In MeOH, EtOH
Storage Conditions -20C*, Desiccated, Protect From Light
Notes *2.5mM solution in acidified MeOH. Store at -80oC for long periods.

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References and Citations

Citations:

  • Oglesby IK, Vencken SF, Agrawal R, Gaughan K, Molloy K, Higgins G, McNally P, McElvaney NG, Mall MA, Greene CM. (2015) "miR-17 overexpression in cystic fibrosis airway epithelial cells decreases interleukin-8 production." Eur Respir J. 46(5):1350-60.
  • Hassan T, Smith SG, Gaughan K, Oglesby IK, O'Neill S, McElvaney NG, Greene CM.(2013) "Isolation and identification of cell-specific microRNAs targeting a messenger RNA using a biotinylated anti-sense oligonucleotide capture affinity technique." Nucleic Acids Res. 41(6):e71.
  • Oglesby IK, Bray IM, Chotirmall SH, Stallings RL, O'Neill SJ, McElvaney NG, Greene CM. (2010) "miR-126 is downregulated in cystic fibrosis airway epithelial cells and regulates TOM1 expression." J Immunol 184(4):1702-9. doi: 10.4049/jimmunol.0902669.
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References:

  • Tannous BA, Kim D-E, Fernandez JL, Weissleder R, Breakefield XO. (2005) "Codon-optimized gaussia luciferase cDNA for mammalian gene expression in culture and in vivo." Molecular Therapy 11(3): 435-443.
  • Kervinen M, Patsi J, Finel M, Hassinen IE. (2004) "Lucigenin and coelenterazine as superoxide probes in mitochondrial and bacterial membranes." Anal Biochem 324: 45-51.
  • Zhao H, Doyle TC, Wong RJ, Cao Y, Stevenson DK, Piwnica-Worms D, Contag CH. (2004) "Characterization of coelenterazine analogs for measurements of Renilla luciferase activity in live cells and living animals." Mol Imaging 3: 43-54.
  • Verhaegent M, Christopoulos TK. (2002) "Recombinant gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization." Anal Chem 74(17): 4378-85.
  • McCapra and Beheshti in "Bioluminescence and Chemiluminescence: Instruments and Applications", 1985, Ed. K. Van Dyke, CRC Press, Boca Raton, FL. pgs. 9-42.
  • Schuster GB, Schmidt SP. (1982) "Chemiluminescence of organic compounds." Adv Phys Org Chem 18: 187.
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