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The cytochome P-450s are a large group of monooxygenase enzymes responsible for the metabolism
of toxins in the body. These enzymes, requiring NADPH as a cofactor and O2 as co-substrate, are located in the
endoplasmic reticulum and are highly concentrated in the liver and small intestine. Additionally, Cytochrome P-450s
are also found in the mitochondrial membrane. P-450 enzymes encompass a highly diverse "superfamily" of hemoproteins,
and metabolic oxidation of chemical compounds is the main function of the cytochrome-mediated monooxygenase or
mixed-function oxidase system. Cytochrome P450 is also used as a marker for the endoplasmic reticulum.
The active site of cytochrome P450 contains the catalyst heme (iron-porphyrin) with a sulfur of cysteine serving as
the fifth iron ligand. Although these enzymes typically have a low turnover rate, their intracellular activity can be
monitored using fluorogenic alkyl ether derivatives of fluorophores. Alkyl ethers of D-Luciferin have also recently
been used in ultrasensitive chemiluminescent assays of cP450 isozymes, with added luciferase. Microsomal dealkylases
are members of this class of enzymes.
| ID
| Name
| CAS
| Unit Size
| Price
| Bulk Price (5+) |
| M1209 |
Resorufin ethyl ether |
5725-91-7 |
5 mg |
123.25 |
98.60 |
| Microsomal dealkylase and Cytochrome P450 enzyme substrate. Releases the red fluorophore resorufin (Abs. 571 nm; Em. 585 nm) upon enzyme reaction. Routinely used to differentiate the various cytochrome P450 isoforms. |
|
| M1166 |
Resorufin 7-O-methyl ether |
5725-89-3 |
5 mg |
112.88 |
90.30 |
| One of the most popular substrates for analysis of microsomal dealkylase and cytochrome P450 enzymes. |
|
| M1231 |
7-Methoxycoumarin |
531-59-9 |
50 mg |
61.45 |
49.16 |
| Fluorogenic Cytochrome P450 and microsomal dealkylase substrate for several isoforms of these enzymes with blue fluorescence emission. |
|
| M0236 |
D-Luciferin, 6'-methyl ether |
3022-11-5 |
5 mg |
100.25 |
80.20 |
| Inhibitor of the firefly luciferase light producing system. Useful in chemiluminescent dual assay systems with both luciferase and cytochrome P450 enzymes. |
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