D-Luciferin, ethyl ester

Product ID: M0906

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Membrane permeable analog of the primary substrate for the firefly luciferase light producing system with 30% higher light intensity.

This membrane permeant derivative of the common firefly luciferase substrate produces 30% higher light intensity. It is extremely useful for in vivo analysis of cloned luciferase activity in whole animal studies.

Technical Data
SKU M0906
CAS Number 135251-85-3
Unit Size 10mg
Alternative Names Ethyl 4,5-dihydro-2[6-hydroxy-2-benzthiazolyl]-4-thiazole carboxylate
Detection Method Luminescence
Molecular Formula C₁₃H₁₂N₂O₃S₂
Molecular Weight 308.38
Storage Conditions -20C, Desiccated
Notes The esterification of the D-luciferin carboxyl group increases the uptake of the substrate by intact mammalian cells in live-cell luciferase assays.

References and Citations


  • "Membrane-permeable Luciferin Esters for Assay of Firefly Luciferase in Live Intact Cells." F.F. Craig, et al. Biochem. J. 276: 637 (1991).
  • "Applications of the Direct Imaging of Firefly luciferase Expression in Single Intact Mammalian Cells Using Charge-coupled Device Cameras." M.R.H. White, et al.Bioluminescence and Chemiluminescence: Current Status. P.E. Stanley and L.J. Kricka (Eds), John Wiley, Chichester, pp. 357-360.
  • "CCD Imaging of Luciferase Gene Expression in Single Mammalian Cells." C.E. Hooper, et al. J. Bioluminescence and Chemiluminescence. 5:123 (1990).
  • "Real-time Analysis of the Transcriptional Regulation of HIV and hCMV Promoters in Single Mammalian Cells." M.R.H. White, et al. J. of Cell Science 108:441 (1995)
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