Dansyl hydrazine

Product ID: M1385

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For use in HPLC labeling of aldehydes and ketones.

The primary amine of dansyl hydrazide can be reversibly coupled to aldehydes and ketones to form Schiff bases that can be reduced to a generate stable derivatives using sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to dansyl hydrazide in aqueous solution using water-soluble carbodiimides such as EDAC. This fluorophore is lipophilic and environmentally-sensitive.

Technical Data
SKU M1385
CAS Number 33008-06-9
Unit Size 100mg
Alternative Names 5-dimethylaminonaphthalene-1-sulfonyl hydrazine
Molecular Formula C₁₂H₁₅N₃O₂S
Molecular Weight 265.33
Storage Conditions 4C, Desiccated, Protect From Light

References and Citations


  • Volpi, Nicola. (2000). "Hyaluronic acid and chondroitin sulfate unsaturated disaccharides analysis by high-performance liquid chromatography and fluorimetric detection with dansylhydrazine." Anal. Biochem.:277(2):19-24.
  • Appelblad, Patrik; Ponten, Einar; Jaegfeldt, Hans; Baeckstroem, Torbjoern; Irgum, Knut. (1997). "Derivatization of Steroids with Dansylhydrazine Using Trifluoromethanesulfonic Acid as Catalyst." Anal. Chem.:69(23):4905-4911.
  • Wu, Hung-Yi; Lin, Jen-Kun. (1995). "Determination of Aldehydic Lipid Peroxidation Products with Dabsylhydrazine by High-Performance Liquid Chromatography." Anal. Chem.:67(9):1603-1612.
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